Influence of the Low-molecule-weight Polysaccharides of Agaricus blazei Murrill on Proliferation and Invasion in Gastric Cancer BGC823 Cells
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Abstract:
The effect of polysaccharides with low molecule weight from Agaricus blazei Murrill (LABP) on proliferation and invasion in gastric cancer cell line BGC823 was observed by WST-1 and Transwell assay, and the expression changes of some related molecules in proteinic level were determined by Western blot, to explore the mechanism of LABP affected on BGC823 cells. Compared with the control group, LABP group cells were significantly declined (P<0.05) in a dose-dependent manner. BGC823 cell inhibition rates by LABP were 33.4% and 69.8%, respectively on LABP concentration of 1 × 10-4g/mL. The cells permeated the membrane of LABP groups were obviously less and the protein expression of Caspase 3 of LABP group was dramatic increased (P<0.05). The expression of MMP 9 was dramatic down-regulated (P<0.05), while E-cadherion had no significant difference (P>0.05). Thus, low-molecule polysaccharide of Agaricus blazei Murrill (LABP) had a dramatic inhibiting effect on growth and invasion in gastric cancer BGC823 cells. LABP inhibited the gastric cancer BGC823 cells growth, possibly by up-regulating the protein expression of Caspase 3 and down-regulating MMP 9.
