Application of Resonance Light Scattering Spectrometry in Assessment of the Potential Toxicity of Bergapten
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Abstract:
DNA intercalation theory was applied in study of the interaction between bergapten which was one kind of flavourings and DNA both in E. coli cells and in vitro by resonance light scattering spectroscopy. The DNA saturation binding value with bergapten was calculated and compared with the saturation values of ethidium bromide, adriamycin, mitoxantrone, and isopsoralen. The results showed that bergapten could partly enter E. coli cells and its abilities to both enter the cells and combine with DNA were lower than those of ethidium bromide. In a neutral environment, the DNA-binding saturation value of bergapten was 0.18, while the saturation values with ethidium bromide, adriamycin, mitoxantrone and isopsoralen were 14.70, 10.58, 3.31, and 0.34, respectively. As a consequence, it appeared that the potential toxicity of bergapten was much lower than those of ethidium bromide, adriamycin, mitoxantrone and a little lower than that of isopsoralen. This research provided technical assistance and reference for quickly testing the potential toxicity of bergapten and flavourings with the similar structure of bergapten.
