Determination of Prodigiosin in Fermentation Broth by HPLC
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Abstract:
Prodigiosin is a multifaceted secondary metabolite with tripyrrole structure, which attracts great interest for its anticancer activities. At present, the main detection method is spectrophotometry. In order to detect prodigiosinin fermentation broth accurately and rapidly， a high performance liquid chromatography（HPLC）method with gradient elution was developed. The best conditions were established as follows: the separation performed on sepax Bio-C18 column (150 mm×4.6 mm, 3 μm) at 35 ℃ with flow rate of 0.6 mL/min. Under the chosen conditions, prodigiosin could be separated well and showed a symmetrical peak shape with acetonitrile-water gradient elution system. The liner range of prodiogisin was 6~150 μg/mL, and the limit of detection was 0.0058 μg/mL. The recovery of standard addition was 95.74~98.41% with precision of 0.92% relative standard deviation (RSD). The proposed HPLC method could be used for the determination of prodigiosinin fermentation broth with high sensitivity, good repeatability and accuracy.
