Preparation of Water-soluble Curdlan Oligose and Determination of Its Activity
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Abstract:
Soluble curdlan oligose with low molecule weight (CRDO) was prepared from curdlan by oxidative degradation of hydrogen peroxide in alkaline homogeneous phase. The effects of NaOH concentration, dosages of H2O2，temperature，and time on curdlan degradation were studied. The optimal hydrolysis conditions were determined as follows: NaOH concentration 2 mol/L, H2O2 dosage 1.5% (V/V), temperature 40 ℃ and reaction time 48 h. The degradative production was examined by paper chromatography and infrared spectrum analysis, which demonstrated that the chemical structure of the hydrolysates had no change. .In addition, its antitumor activity antibacterial activity and effects on growth performance of Penaeus japonicus were studied. The results showed that it could inhibit the growth of S-180 sarcoma. When CRDO dosage was 50 mg/kg of a rat, the antitumor acitivity was 48.73%. Meanwhile, it could improve the survival rate of Penaeus japonicas, and make the PO activity up to the highest of 2.96 U/mg when CRDO dosage was 400 mg/kg of feed. The antimicrobial tests indicated that the water-soluble CRDO had certain antibacterial effect on staphylococcus aureus and had weak antimicrobial effect on E. coli, but no inhibition on angel yeast.
