Detection for Genetically Modified Composition of Soybean, Rice and Corn by Thin-film Biosensor Chips
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Abstract:
Nine exogenous DNA fragement were selected as target genes. Their primers and probes were designed and synthesized, and then the probes were immobilized in the Thin-Film Biosensor Chips. PCR was used to amplify the target sequence in food sample DNA, and then the Biochip was hybridized with PCR product. The chip displayed the hybridization result. The method can detect 5 kinds of common modified plant. It was efficient, accurate, simple, high-throughput, practical, sensitive (0.1%), and got rid of the dependence of fluorescence scanner.
