Medium Optimization for Liquid Fermentation of Ganoderma lucidum Producing Intracellular Polysaccharides
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Abstract:
The culture of Ganoderma lucidum mycelium was researched for production of the mycelial intracellular polysaccharides through liquid fermentation method, using mycelial biomass and mycelial intracellular polysaccharide as the indicator. And the influence of carbon sources, nitrogen sources and trace elements on the growth of Ganoderma lucidum mycelium and polysaccharide yield were evaluated through single factor and orthogonal experiment. The result showed that the perfect culture medium composition of producing mycelium with Ganoderma lucidum mycelium liquid fermentation was maltose 2.0%, yeast extract 2.0%, ZnSO4 0.015%, VB1 0.10%, KH2PO4 0.2%, MgSO4 0.05% and pH 6.0. Under the optimum conditions, the mycelial polysaccharide yield was up to 6.64%.
