Construction and EST Analysis of cDNA Library of the Rosy Barb's 1-6 Somite Develomental Stage
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Abstract:
Abstract In the present study a cDNA library of the rosy barb 1-6 somite stage was constructed. The titer of the cDNA library was estimated as~1.4×106 pfu/mL with the recombination rate above 80%. 112 clones with insert≥1kb to sequence were randomly picked and 83 independent sequences were got. BLASTX analysis of the 83 cDNAs showed that the amino acid sequences of the polypeptides deduced from 73 of the clones were strong matches(P<10-10) to those of previously identified proteins, while the other (10 cDNAs) showed no similarity using the present sequence determination and database analysis. On the basis of these results, 2 cDNAs for genes were identified being associated with Cytoskeleton and motility and 6 genes for proteins were related to Signal transduction. And 5 genes were for energy conversion and 8 genes were for protein sythesis. In addition, 3 gens were for development related proteins, and 2 genes were forimmunal proteins. And 27 genes were for hypothetical protein and 20 genes were for others. This study provided a preliminary view of the gene information involved in the development of the rosy barb 1-6 somite stage.
