采用顶空-固相微萃取-气相色谱质谱联用法（HS-SPME-GC-MS）对4种柑橘果皮精油挥发性物质的组成和含量进行分析鉴定，结合相对气味活度值（Relative Odor Activity Value，ROAV）对4种柑橘果皮精油中的关键香气成分进行分析，以期为柑橘果皮精油品质评价与发掘特殊香气柑橘精油提供依据。结果表明，4种柑橘果皮精油共鉴定出62种挥发性成分，包括13种单萜、14种倍半萜、5种烷烃、12种醇类、8种醛类、4种酯类、3种酮类、2种醚类和1种酸类。4种柑橘果皮精油均以单萜化合物相对含量最高（83.00%~91.93%）。按照化合物组成，可将4种柑橘分为2个化学类型：柠檬烯/γ-萜品烯型和柠檬烯/3-蒈烯型。ROAV法从4种柑橘果皮精油中筛选出24种关键香气物质，其中北京柠檬果皮精油中风味贡献最大的物质为紫苏醛，关键香气物质共10种；温州蜜柑、酸橙和枳雀果皮精油中风味贡献最大的物质均为芳樟醇，关键香气物质分别为12种、20种和20种。这些关键香气物质对柑橘果皮精油的香气品质起着重要的作用。

To provide the basis for the quality evaluation of citrus peel essential oils and the discovery of special aroma citrus essential oils, the composition and content of volatile substances in the essential oil from the fruit peels of four citrus varieties were analyzed by headspace-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS), along with relative odor activity values (ROAVs) of the key aroma components in four kinds of citrus peel essential oils. The results showed that a total of 62 volatile compounds were identified from the essential oils of four citrus cultivars, including 13 monoterpene, 14 sesquiterpene, 5 alkanes, 12 alcohols, 8 esters, 4 aldehydes, 3 ketones, 2 ethers and 1 acid. Among the four citrus peel essential oils, the content of monoterpene (83.00%~91.93%) was the highest. According to the compound composition, the four citrus species can be divided into two chemical types: limonene/γ-terpinene type and limonene//3-carene type. Twenty-four key aroma compounds were screened from four citrus peel essential oils by ROAV, among which perillaldehyde was the most important flavor contributor in the essential oils of Citrus meyerii Y. Tan., and there were 10 key aroma substances. Linalool was the most important flavor contributor in the essential oils of Citrus unshiu Marc., Citrus aurantium L. and Citrus wilsonii Tanaka, and there were 12, 20 and 20 key aroma components, respectively. These key aroma components play important roles in the aroma quality of citrus peel essential oils.

陕西省科技厅项目（2022NY-141）；陕西省教育厅协同中心项目（21JY006）；陕西理工大学秦巴生物资源与生态环境重点实验室（培育）“市校共建”科研专项（SXC-2305）