该文以钼酸铵、鸡蛋壳、十六烷基三甲基溴化铵为原材料，通过改变煅烧温度制备了性能更为优良的CaMoO4，并以钼酸钙、硫酸铜、硼氢化钠为原料，制备了CuO/CaMoO4 复合抗菌剂，同时研究了CuO/CaMoO4 复合抗菌剂的用量、培养时间、铜含量对抗菌活性的影响，以及CuO/CaMoO4 复合抗菌剂在光反应和暗反应条件下对大肠杆菌（Escherichia coli，E. coli）的抑菌效果。实验结果表明，在650 ℃煅烧下得到的CuO/CaMoO4 复合抗菌剂的抗菌性能最好，铜含量为1.20% 时，光照条件下和黑暗条件下的抑菌率均能达到100%。含有CuO/CaMoO4 复合抗菌剂的保鲜膜抑菌试验表明，CuO/CaMoO4 复合抗菌剂可以有效抑制食品表面的大肠杆菌，食品经贮藏4 d 后，抑菌率仍为100%。CuO/CaMoO4 复合抗菌剂生物相容性好。CuO/CaMoO4 复合抗菌剂能快速高效抑制大肠杆菌，保障食品安全，为食品贮存行业提供了新的思路。

In this paper, ammonium molybdate, eggshell and cetyltrimethyl ammonium bromide were used as raw materials to prepare CaMoO4 with improved performance by adjusting the calcination temperature. Additionally, a CuO/CaMoO4 composite antibacterial agent was synthesized using calcium molybdate, copper sulfate, and sodium borohydride as raw materials. The effects of CuO/CaMoO4 antibacterial dosage, culture time, and copper content on antibacterial activity and the antibacterial effect of CuO/CaMoO4 on Escherichia coli (E. coli) were studied under light and dark reaction conditions. The CuO/CaMoO4 composite antibacterial agent synthesized at 650 ℃ exhibited the best antibacterial efficacy. Moreover, when the copper content was 1.20%, the antibacterial rate could reach 100% under light and dark conditions. Bacteriostatic testing of plastic wrapping material containing CuO/CaMoO4 compound antibacterial agent showed that the agent could effectively inhibit E. coli on food surfaces, and the antibacterial rate remained at 100% even after four days of storage. Furthermore, the CuO/CaMoO4 antibacterial compound displayed excellent biocompatibility. Overall, the compound CuO/CaMoO4 antibacterial agent demonstrated rapid and efficient inhibition of E. coli, thereby ensuring food safety and offering novel insights for food storage practices.

中央引导地方科技发展专项（YDZX2021127）；山东省高等学校青创人才引育计划-食品加工技术技能创新团队建设项目（2019.133）