该文探究毛菊苣醇提物对糖尿病小鼠（db/db小鼠）肠道菌群的影响。该研究以m/m小鼠为正常对照组（CON组），db/db小鼠随机分为模型组（MOD组）、二甲双胍组（MET组）、毛菊苣醇提物低剂量组（CGL组）、毛菊苣醇提物高剂量组（CGH组）。灌胃给药8周，对小鼠体质量、空腹血糖、肝脏总胆固醇（TC）、甘油三酯（TG）进行分析，对小鼠粪便进行16S rRNA测序分析，探究毛菊苣醇提物对小鼠肠道菌群的影响。毛菊苣醇提物高剂量组可调节db/db小鼠体质量及血糖，显著降低db/db小鼠TC、TG水平（P<0.05）；测序结果显示CON组、MOD组、CGH组三组小鼠肠道菌群情况存在差异，毛菊苣醇提物给药可改善db/db 小鼠的肠道菌群失调，上调db/db小鼠肠道菌群中有益菌双歧杆菌属（Bifidobacterium）、Romboutsia、普雷沃氏菌属（Prevotella）菌群相对丰度。研究证明了毛菊苣醇提物给药能调节db/db小鼠肠道菌群，通过提高有益菌相对丰度调节糖脂代谢，可为新疆地产毛菊苣药材资源的开发利用提供全新的研究思路。

The effects of ethanol extracts obtained from Cichorium glandulosum on the intestinal microbiota of diabetic mice (db/db mice) were investigated. In this study, m/m mice served as the normal control group (CON group), while db/db mice were randomly divided into the model group (MOD group), metformin-treated group (MET group), lowdose ethanol extract-treated group (CGL group), and high-dose ethanol extract-treated group (CGH group). After intragastric administration of ethanol extracts from Cichorium glandulosum for eight weeks, the body weight, fasting blood glucose levels, liver total cholesterol (TC) levels, and liver triglyceride (TG) content in mice were analyzed. Additionally, 16S rRNA sequencing analysis was performed on fecal samples of mice to investigate the changes in their intestinal microbiota after the treatment. It was discovered that the high-dose ethanol extract can regulate the body weight and blood glucose levels of db/db mice and significantly reduce the levels of TC and TG in db/db mice (P<0.05). In the meantime, the 16S rRNA sequencing results showed noticeable differences in the intestinal microbiota among the CON, MOD, and CGH groups. The administration of ethanol extracts from Cichorium glandulosum improved the intestinal dysbiosis in db/db mice and upregulated the relative abundance of beneficial bacteria such as Bifidobacterium, Romboutsia, and Prevotella in the intestinal microbiota. This study has shown that the ethanol extract from Cichorium glandulosum can regulate the intestinal microbiota of db/db mice and regulate glucose and lipid metabolism by increasing the relative abundance of beneficial bacteria. This can provide new insights for the development and utilization of Cichorium glandulosum from Xinjiang as a medicinal resource.

国家自然科学基金资助项目（81760753）；自治区天山英才青年拔尖人才项目（2022TSYCCX0104）