萝卜硫苷（Glucoraphanin，RAA）是广泛存在于十字花科植物中的硫代葡萄糖苷，其代谢产物具有防癌抗癌等功能。但从十字花科植物中提取的RAA纯度较低，降低了其利用率，因此需要对RAA纯化。当前纯化RAA的主要技术为色谱纯化技术，但该技术存在回收率低以及二次污染等问题。此外，因膜纯化技术是分离纯化糖苷类物质最常用的方法，具有设备和操作简单以及无二次污染等特点，故该技术同样是纯化RAA的潜在技术。因此， 色谱纯化与膜纯化联合技术可实现RAA的有效回收并提高RAA的纯度，有效规避色谱纯化技术所造成的RAA回收率低及二次污染的缺点。因此，该文主要讨论了目前RAA主要的纯化方法，分析了色谱和膜纯化技术在RAA纯化中联合应用的可行性，为RAA的高效纯化提供方法参考。

Glucoraphanin (RAA) is a glucosinolate widely found in cruciferous plants, the metabolites of which have been established to have anti-cancer effects. However, the purity of RAA extracted from cruciferous plants is low, which limits its effective utilization, and consequently, it is necessary to develop an effective process for RAA purification. Although the main technique currently used for the purification of RAA is chromatographic purification, this procedure has notable drawbacks, including low recovery rates and secondary contamination. As an alternative procedure, membrane purification, which is the most commonly used method for the separation and purification of glycosides, requires only simple equipment and entails simple operation. Moreover, it is less prone to secondary contamination. Accordingly, this separation procedure is considered a potential technique for the purification of RAA. It is speculated that combined chromatographic and membrane purification could facilitate the effective recovery of RAA and enhance the purity of the recovered product, thereby overcoming the problems of low recovery and secondary contamination of RAA associated with the sole use of chromatographic purification. This study focuses mainly on the main methods currently used for the purification of RAA and assesses the feasibility of the combined application of chromatography and membrane purification techniques for RAA purification, thereby providing a methodological reference for the efficient purification of RAA.

国家自然科学基金青年基金项目（32302104）；中国劳动关系学院校级教改项目（JG23016）