该研究以1株高毒力持留基因型（Hypervirulent persistent genotypes, HVPG）小肠结肠炎耶尔森氏菌Y2844为宿主菌，从广州市污水样本中分离纯化一株新型小肠结肠炎耶尔森氏菌烈性噬菌体vB_YenP_WW1（WW1）。WW1的全基因组序列长度为39589 bp，同噬菌体Escherichia phage P483的核苷酸序列相似性最高（92.77%）。耐受性实验结果表明，WW1能耐受较宽的温度（4~50℃）和pH值（5~11）；消杀实验结果表明，WW1在28℃，8 h内显著抑制LB肉汤中小肠结肠炎耶尔森氏菌的生长。在4℃下作用24 h后，WW1可将猪肉表面小肠结肠炎耶尔森氏菌的数量由3.82 lg CFU/mL降低至2.67 lg CFU/mL，将牛奶中小肠结肠炎耶尔森氏菌的数量由5.41 lg CFU/mL降低至检测限以下。同时，WW1还可有效抑制小肠结肠炎耶尔森氏菌在聚乙烯及不锈钢表面形成的生物膜。综上所述，该研究分离的噬菌体WW1耐受性强，对小肠结肠炎耶尔森氏菌的生长及生物膜均具有显著的抑制作用，有望成为一种抑菌制剂用于防控食品及食品加工车间中污染的小肠结肠炎耶尔森氏菌。

In this study, one hypervirulent persistent genotypes (HVPG) Yersinia enterocolitica (Y. enterocolitica) strain Y2844 was selected as the host bacteria, and one lytic bacteriophage, vB_YenP_WW1 (WW1), was isolated from sewage samples. Genome sequence analysis revealed that the sequence of phage WW1 was 38589 bp long, with the highest nucleotide sequence similarity (92.77%) with Escherichia phage P483. Phage WW1 demonstrated tolerance to a wide range of temperatures (4~50℃) and pH values (5~11), and effectively inhibited Y. enterocolitica growth in LB broth at 28℃ up to 8 h. After 24 h phage treatment at 4℃, viable counts of Y. enterocolitica in raw pork could be reduced from 3.82 lg CFU/mL to 2.67 lg CFU/mL, and the amount of Y. enterocolitica in milk could be reduced from 5.41 lg CFU/mL to below the detection limit. Furthermore, phage WW1 exhibited substantial inhibition of biofilm formation on polyethylene and stainless steel. In summary, phage WW1 exhibits strong tolerance and preventive effects against Y. enterocolitica and its biofilm. Our results suggest that phage WW1 could be used as a promising antimicrobial agent against Y. enterocolitica in food matrices and food processing factories.

国家重点研发计划项目（2023YFD1801000）；广东省基础与应用基础重大项目（2020B0301030005）；广东特支计划（2021TQ06N119）