探究白块菌多糖（Tuber panzhihuanense Polysaccharide，TPP）的理化特性，研究其对运动疲劳小鼠肝脏氧化损伤的保护作用。采用超声辅助热水提取粗多糖，柱层析分离纯化并测定其分子量和单糖组成，构建小鼠运动疲劳模型，测定相关生化指标以探究TPP对运动疲劳小鼠肝脏的保护作用。结果显示，TPP分离纯化得到TPP-A和TPP-B两个主要组分，其分子质量分别为23.74和46.81 ku，TPP-A含有甘露糖和葡萄糖，TPP-B含有甘露糖、葡萄糖和葡萄糖醛酸；与过度运动模型组（E-CK）相比，TPP组明显减少了小鼠肝细胞中的炎症因子，降低了血清中谷丙转氨酶、谷草转氨酶水平以及肝组织中活性氧、丙二醛含量（P<0.05），提高了肝组织中抗氧化酶活性，其低、中、高剂量组超氧化物歧化酶活性分别升高了18.15%、39.73%、59.06%（P<0.05），过氧化氢酶活性分别升高了49.91%、86.40%、127.80%（P<0.05），谷胱甘肽过氧化物酶活性分别升高了16.00%、24.01%、38.87%（P<0.05）。研究表明TPP具有提高小鼠肝脏组织抗氧化酶活性的作用，其对运动疲劳小鼠肝脏损伤的保护作用可能与降低细胞的氧化应激水平有关。

The physicochemical properties of Tuber panzhihuanense polysaccharides (TPP) and their protective effects on liver oxidative damage in exercise-fatigue mice were explored. U ltrasound assisted hot water extraction was adopted to extract the crude polysaccharides from T. panzhihuanense, while column chromatography was applied to separate and purify them and determine their molecular weights and monosaccharide compositions. Exercise-fatigue mouse models were constructed. Relevant biochemical indicators were measured to explore the protective effects of TPP on the livers of exercise-fatigue mice. The results showed that TPP-A and TPP-B were obtained after separation and purification of TPP. The molecular weight of TPP-A equals 23.74 ku, and it is composed of mannose and glucose. The molecular weight of TPP-B is 46.81 ku, and it consists of mannose, glucose, and glucuronic acid. Comparison of the TPP treatment group and the excessive exercise model group (E-CK) showed that TPP significantly reduced inflammatory factors in mice liver cells, the levels of glutamate-pyruvate transaminase and glutamic oxaloacetic transaminase in serum, and the contents of reactive oxygen species (SOD) and malondialdehyde in liver tissues (P<0.05), but significantly increased the activity of antioxidant enzymes in liver tissues. In the low-, medium-, and high-dose TPP groups, the SOD activity increased by 18.15%, 39.73%, and 59.06%, respectively (P<0.05), whereas CAT activity increased by 49.91%, 86.40%, and 127.80%, respectively (P<0.05). Furthermore, GSH activity increased by 16.00%, 24.01%, and 38.87% in the low-, medium-, and high-dose TPP groups, respectively (P<0.05). The results showed that TPP enhances the activity of antioxidant enzymes in mouse liver tissues, and that its protective effects on liver injury in fatigue mice may be related to the reduction in the level of oxidative stress in cells.

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