利用自由基清除能力评价发酵祛湿汤（Fermented Qushi Decoction，FQD）的体外抗氧化活性；结果显示，与未发酵祛湿汤（Qushi Decoction，QD）相比，高剂量FQD组的总抗氧化能力显著提高，其中1,1-二苯基-2-三硝基苯肼自由基清除率、羟自由基清除率和总还原能力分别提高了24.38%、11.98%、11.76%。FQD对线虫总产卵量无显著影响，并能促进线虫的运动；与QD相比，FQD干预后线虫平均寿命延长了8.02%~26.36%；在热应激条件下，FQD组最长存活时间提高了25%，在氧化应激下，FQD组线虫的生存时间较QD提高了16.67%。此外，FQD作用后，线虫体内抗氧化酶活性显著增强（P<0.05），脂褐质的堆积水平降低。综上，FQD通过提高体外抗氧化能力，延长线虫寿命，提升抵抗应激能力和抗氧化酶活性来延缓线虫的抗衰老作用。该研究初步表明发酵祛湿汤具有抗衰老作用，为保健品开发和利用提供理论基础。

The in vitro antioxidant activity of fermented qushi decoction (FQD) was evaluated based on the free radical scavenging capacity. The results showed that the total antioxidant capacity of the high-dose FQD group was significantly higher than that of the unfermented qushi decoction (QD) group. In particular, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging rates and the total antioxidant capacity significantly increased by 24.38%, 11.98%, and 11.76%, respectively, in the high-dose FQD group. FQD showed no significant effects on the total egg production of Caenorhabditis elegans. However, the average lifespan of C. elegans increased by 8.02% to 26.36% after FQD intervention. Under heat stress and oxidative stress conditions, the maximum survival time of C. elegans was extended by 25% and 16.67% in the FQD groups, respectively. In addition, the antioxidant enzyme activity of C. elegans was significantly enhanced (P<0.05) and the level of lipofuscin accumulation was reduced after FQD intervention. In conclusion, FQD provides anti-aging effects on C. elegans by increasing the in vitro antioxidant capacity, prolonging the lifespan of C. elegans, enhancing stress resistance, and improving the antioxidant enzyme activity. This study tentatively indicates that FQD has anti-aging effects, and the findings provide a theoretical basis for the development and utilization of related nutraceuticals.

财政部和农业农村部：国家现代农业产业技术体系资助（CARS-21）