灰葡萄孢菌侵染是导致采后草莓病害发生的关键原因。该研究以草莓为研究对象,探究低频极化电场(Low-frequency polarized electric field,LFPEF)对采后草莓贮藏期间的抗病性能及品质的影响。结果表明,LFPEF处理可有效延缓草莓失重,维持果实硬度及可溶性固形物含量,降低灰霉菌病斑直径,提高其贮藏品质。同时,LFPEF通过提高草莓果实体内APX、GSH、H2O2的含量,降低CAT活性,提高SOD、APX、GR活性,激活了机体抗氧化系统。此外,LFPEF处理还可以有效维持草莓原果胶及总果胶含量,降低CEL、β-GAL、PME、PG活性,从而强化细胞壁结构以抵御病害侵染。综上所述,LFPEF处理可通过激活采后草莓贮藏过程中抗氧化系统,降低细胞壁降解酶活性,维持果胶含量,提高采后草莓抗病性,达到维持果实营养品质,延长货架期的目的。

Botrytis cinerea infection is the key cause of postharvest strawberry disease. Using strawberries as the research object, we explored the effects of Low-frequency polarized electric field (LFPEF) on the disease resistance and quality of post-harvest strawberries during storage. The results showed that LFPEF treatment could effectively delay the weight loss of strawberry, maintain the fruit hardness and soluble solid content, reduce the diameter of gray mold spot and improve the storage quality of strawberry. The application of LFPEF simultaneously enhances the levels of ascorbic acid, glutathione, and H2O2 in strawberry fruit while reducing catalase activity. Additionally, it improves the activities of superoxide dismutase, ascorbate catalase, and glutathione reductase, thereby enhancing the plant antioxidant capacity. Moreover, LFPEF treatment can effectively maintain the contents of propectin and total pectin of strawberry, and reduce the activities of cellulase, β-galactosidase, pectin methylesterase and polygalacturonase, thus strengthening the cell wall structure to resist disease infection. In summary, LFPEF treatment can activate the antioxidant system of postharvest strawberry and reduce the activity of cell wall degrading enzyme to maintain the pectin content, thus achieving the purpose of maintaining the nutritional quality of fruit and extending the shelf life.

江苏省重点研发计划；国家自然科学基金；江苏省农业科技自主创新资金