[关键词]
[摘要]
探讨辣木籽蛋白酶解物(Moringa oleifera Seed Protease Hydrolysate,MSPH)对小鼠肠道黏膜炎的改善作用。提取辣木籽蛋白并进行酶解,冷冻干燥得到MSPH ;随机将雄性BALB/c 小鼠分为空白对照(Con)组、模型(Mod)组和MSPH 组(800 mg/kg•BW),每组6 只,腹腔注射5-FU 诱导小鼠肠道黏膜炎,MSPH 灌胃干预。每天记录小鼠体质量;通过HE 染色观察肠道组织形态学并计算绒毛高度/ 隐窝深度;酶联免疫法测定小鼠血清(INF-γ、SIgA、ROS、G-CSF、ET、D-LA)和小肠组织(IL-1β、IL-6、MDA、MPO、TNF-α)含量变化;实时荧光定量聚合酶链式反应检测小肠组织ZO-1 和Occludin mRNA 表达量。与Mod 组相比,MSPH 组小鼠在实验后期体质量显著提高(P=0.001 1),脾脏和胸腺指数分别增加16.22% 和34.69%,肠绒毛高度/ 隐窝深度增加25.88%,血清中INF-γ、ROS、G-CSF、ET、D-LA 含量分别降低16.85%、17.50%、14.22%、8.28%、9.27%,SIgA 含量升高23.07%,小肠组织中IL-1β、IL-6、MDA、MPO、TNF-α 含量分别降低9.72%、11.23%、9.74%、7.29%、5.17%,肠道组织ZO-1 和Occludin mRNA 表达量增加121.64% 和29.35%。因此,MSPH 可以通过减轻炎症和氧化应激及降低肠道通透性改善5-FU 诱导的小鼠肠道黏膜炎。
[Key word]
[Abstract]
The ameliorating effects of Moringa oleifera seed protein hydrolysate (MSPH) on intestinal mucositis in mice were investigated. Moringa oleifera seed protein was extracted, enzymolized, and freeze-dried to obtain MSPH. Male BALB/c mice were randomly divided into the blank control (Con) group, the model (Mod) group, and the MSPH treatment group with six mice in each group. 5-fluorouracil (5-FU) was intraperitoneally injected to induce intestinal mucositis in mice, and MSPH (800 mg/kg•BW) was administered orally for intervention. The weights of the mice were recorded daily. The intestinal histopathology was observed using HE staining, and the villus height/crypt depth was calculated. The concentrations of INF-γ, SIgA, ROS, G-CSF, ET, and D-LA in serum and those of IL-1β, IL-6, MDA, MPO, and TNF-α in the small intestine were determined using enzyme-linked immunosorbent assay. The mRNA expressions of ZO-1 and occluding in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction. Compared to the mice in the Mod group, the body masses of those in the MSPH group significantly increased (P=0.001 1) in the later stage of the experiment, while their spleen and thymus indices increased by 16.22% and 34.69%, respectively. The villus height/crypt depth increased by 25.88% in the MSPH group. The INF-γ, ROS, G-CSF, ET, and D-LA contents decreased by 16.85%, 17.50%, 14.22%, 8.28%, and 9.27%, respectively, while the SIgA content increased by 23.07% in the MSPH group compared to that in the Mod group. The contents of IL-1β, IL-6, MDA, MPO, and TNF-α decreased by 9.72%, 11.23%, 9.74%, 7.29%, and 5.17%, respectively, while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64% and 29.35%, respectively, in the MSPH group. Thus, 5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation, oxidative stress and intestinal permeability.
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[基金项目]
国家自然科学基金项目(31960508);国家木薯产业技术体系“辣木产品加工岗位”(CARS-11-YNTY);云南绿色食品国际合作研究中心项目(省科技厅)(2019ZG00905);云南省重大科技专项计划(202002AA100005)