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[摘要]
该文通过HepG2高脂细胞模型,研究植物乳植杆菌dy-1发酵大麦麸皮提取物(FBBE)对细胞脂质沉积及氧化应激的影响。结果显示,大麦麸皮经植物乳植杆菌dy-1发酵后,其蛋白含量未发生显著变化,但52 ku左右较大分子量的蛋白基本被完全水解,20 ku ~35 ku的蛋白显著增加,总糖含量降低了约28%,总酚含量增加了约42%;50 μg/mL的FBBE使高脂细胞的TG含量降低了20.30%,5,10和15 μg/mL的发酵大麦麸皮提取物中多酚组分(FBBE-PP)使细胞TG水平分别降低了16.97%,27.41%和34.92%,同时使细胞内脂滴数量显著减少,说明多酚组分是缓解细胞脂质沉积的有效成分;添加10 μg/mL的FBBE-PP后,细胞内活性氧(ROS)含量相比模型组降低了23.45%,超氧化物歧化酶(SOD)活性升高了40.80%;FBBE-PP可上调细胞ppar-α和cpt-1α的表达,下调scd-1和acc-1的表达缓解细胞脂质沉积;并上调nrf2、ho-1、sod1和cat等的表达增强细胞抗氧化应激能力。综上所述,植物乳植杆菌dy-1的发酵有助于提升大麦麸皮的降脂及抗氧化活性,其多酚组分对高脂诱导的细胞脂质沉积和氧化应激均具有显著的缓解作用。
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[Abstract]
In this paper, barley bran was fermented by Lactiplantibacillus plantarum dy-1 (Lp. plantarum dy-1) and the effects of fermented barley bran extract (FBBE) on lipid deposition and oxidative stress of HepG2 cells were studied. The results showed that, the protein content of barley bran did not change significantly after fermentation with Lp. plantarum dy-1, but the protein with Mw 52 ku in barley bran was almost completely hydrolyzed and the protein of 20 ku ~35 ku was significantly increased, while the total carbohydrates content was reduced by about 28% and the total phenolics content was increased by about 42%. The TG content was decreased by 20.30% after 50 μg/mL of FBBE treatment, while 5 ,10 and 15 μg/mL of polyphenols in fermented barley bran extract (FBBE-PP) decreased the TG levels by 16.97%, 27.41% and 34.92%, respectively. At the same time, FBBE-PP could reduce the lipid droplets in cells significantly, indicating that polyphenols could alleviate lipid deposition in cells. After 10 μg/mL of FBBE-PP treatment, the cellular ROS level decreased by 23.45% compared with the model group, and the SOD activity increased by 40.80%. The FBBE-PP could up-regulate the expressions of ppar-α and cpt-1α, down-regulate the expressions of scd-1 and acc-1, while the anti-oxidative stress ability was enhanced by its up-regulations on the expressions of nrf-2, ho-1, sod1 and cat. In conclusion, the fermentation of Lp. plantarum dy-1 could improve the lipid-lowering and antioxidant activities of barley bran, and its polyphenols could significantly alleviate the lipid deposition and oxidative stress in cells.
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[基金项目]
江苏省重点研发计划;镇江市重点研发计划;盐城市重点研发计划