利用肥胖小鼠模型探讨动物双歧杆菌CP-9（Bifidobacterium animalis CP-9，CP-9）和罗伊氏乳杆菌ADR-1（Limosilactobacillus reuteri ADR-1，ADR-1）对体质量、体脂分布和血脂的影响。高脂饲料喂养的肥胖小鼠随机分为高脂饮食对照组（High-fat Diet，HFD）、CP-9组、ADR-1组，正常饮食组（Normal Diet，ND）普通饲料喂养，每组12只，连续灌胃10 w。测量小鼠体质量、体成分，观察体脂肪分布、肝组织病理变化，检测血液生化指标，测定肝脏组织脂肪酸合成酶（Fattty Acid Synthase，FAS）和过氧化物酶体增殖物激活受体γ（Peroxisome Proliferator-activated Receptor-γ，PPARγ）基因的蛋白表达。干预结束时，与HFD相比，CP-9和ADR-1组小鼠体质量（-18.82%，-18.82% vs HFD）、腹腔和皮下脂肪、血清低密度脂蛋白胆固醇（Low-density Lipoprotein Cholesterol，LDL-C）、甘油三酯（Triglyceride，TG）、谷丙转氨酶（Alanine Aminotransferase，ALT）显著降低（P<0.05）。CP-9、ADR-1 组肝脂肪变性及肝损伤较HFD组明显改善，且肝脏FAS基因的蛋白表达减少（-53.22%，-53.22% vs HFD)，PPARγ基因的蛋白表达增加(571.43%，714.29% vs HFD)。综上，CP-9和ADR-1干预可有效减轻肥胖小鼠体质量、腹腔和皮下脂肪，改善肝脂肪变性及肝损伤，可能是通过调节肝脏FAS和PPARγ基因的蛋白表达降低TG、LDL-C从而改善脂肪代谢异常。

To explore the effects of Bifidobacterium animalis CP-9 (CP-9) and Limosilactobacillus reuteri ADR-1 (ADR-1) on weight, fat distribution and blood fatin obese mice. The obese mice fed with high fat diet were randomly divided into model control group (High-fat Diet, HFD), CP-9 group, ADR-1 group. The mice in blank control group (Normal Diet, ND) were fed with normal diet. The body weight and body composition of mice were measured, the distribution of body fat and the pathological changes of liver tissue were observed, the blood biochemical indexes were measured, and the protein expressions of fatty acid synthase (FAS) and peroxisome proliferator-activated receptor γ (PPARγ) in liver tissue were determined. At the end of the intervention, compared with HFD, the body weight (-18.82%, -18.82% vs HFD), abdominal and subcutaneous fat, serum low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and alanine aminotransferase (ALT) of mice in theCP-9 and ADR-1 groups decreased significantly (P<0.05). Liver steatosis and liver injury in CP-9 and ADR-1 groups were significantly improved compared with HFD group, and the protein expression of FAS gene in liver decreased (-53.22%, -53.22% vs HFD), while the protein expression of PPAR γ gene increased (571.43% vs HFD 714.29%). In summary, the intervention of CP-9 andADR-1 can effectively reduce the body weight, abdominal and subcutaneous fat of obese mice, improve hepatic steatosis and liver injury, probably by regulating the protein expression of FAS and PPAR γ genes in liver to reduce TG and LDL-C so as to improve the abnormality of fat metabolism.