该研究以佤药远志（娘母良）为试材，研究其改善睡眠的功效。采用高效液相色谱法（HPLC）检测佤药远志醇提物的主要活性成分及含量，以斑马鱼为实验动物，建立戊四唑（PTZ）斑马鱼失眠模型。实验设置正常对照组、模型组和处理组，处理组设置5个质量浓度，分别为125、250、500、1 000、2 000 μg/mL，28 ℃处理1 d后，观察统计各组斑马鱼死亡数量和死亡率，测定远志醇提物对正常斑马鱼的最大耐受浓度（Maximum Talerated Concentration，MTC）；根据MTC给予PTZ模型斑马鱼不同质量浓度的远志醇提物，设置阳性对照组（褪黑素组）和正常对照组，通过行为分析仪检测斑马鱼的觉醒活动时间和觉醒活动量。结果表明，MTC为1 000 μg/mL；观察时间内，阳性褪黑素组改善睡眠作用为29.00%，远志醇提物4个质量浓度处理组（125、250、500、 1 000 μg/mL）改善睡眠作用依次为6.97%、22.78%、33.14%、47.26%，其中质量浓度为500 μg/mL和1 000 μg/mL的远志醇提物改善睡眠作用明显优于阳性褪黑素组。以上结果显示远志醇提物对PTZ诱导的斑马鱼失眠症状具有一定的改善作用，但是在一定浓度范围内，呈剂量依赖性，浓度越高，作用越明显。

The effect of different concentrations of Polygala tenuifolia alcohol extracts on sleep improvement in zebrafish was investigated. High-performance liquid chromatography (HPLC) was used to determine the primary active components and their concentrations in P. tenuifolia alcohol extracts. The experimental group was treated with extracts at concentrations of 125, 250, 500, 1 000, and 2 000 μg/mL. After treatment for 1 day at 28 ℃, the number of zebrafish deaths and the mortality rate of zebrafish in each group were observed and counted, and the maximum tolerated concentration (MTC) of samples was determined. According to the MTC, the treatment groups were treated with various concentrations of P. tenuifolia alcohol extracts. In addition to other zebrafish insomnia models, a positive control group (melatonin group) and normal control group were also established. The duration and amount of activity of zebrafish in the awake state were established using a behavior analysis system. MTC was set at 1 000 μg/mL. During the observation period, the sleep improvement effect of the positive melatonin group was 29.00%, and the sleep improvement effects of the four concentration treatment groups (125, 250, 500, 1 000 μg/mL) were 6.97%, 22.78%, 33.14% and 47.26%, respectively. The sleep improvement effect of P. tenuifolia alcohol extract at concentrations of 500 μg/mL and 1 000 μg/mL were significantly superior than that of the positive melatonin group. The findings of this study show that P. tenuifolia alcohol extracts exhibited a dose-dependent improvement effect on PTZ-induced insomnia symptoms in zebrafish, with a more pronounced effect observed at higher concentrations.

国家自然科学基金项目（51973121）；深圳市协同创新专项（GJHZ20210705141800002）