[关键词]
[摘要]
为促进东北地区浆果相关产品的开发与利用,以东北9种种植范围广且常见的浆果为研究对象,测定分析浆果中总酚、总黄酮和总花色苷含量,通过高效液相色谱法测定浆果中酚类单体的种类和含量;通过测定DPPH·自由基清除能力、ABTS+?自由基清除能力、透明质酸酶抑制能力和抑制白蛋白变性能力评价浆果抗氧化活性和抗炎活性,并进行相关性分析。结果显示,红豆越橘和蓝靛果中总酚、总黄酮和总花色苷含量较高,分别为2.83和2.42 g(GAE)/100g DW、0.99和2.09 g(Rutin)/100g DW、104.33和414.73 mg/100g DW,且二者具有较强的抗氧化活性和抗炎活性。相关性结果表明,总酚含量决定浆果的抗氧化活性和抗炎活性。在9种浆果中鉴定并定量19种单体酚,绿原酸、隐绿原酸、熊果苷和黄岑素均在浆果中检出,绿原酸和隐绿原酸是大部分浆果中主要的酚类单体,红豆越橘中单体酚种类最多,蓝靛果中单体酚含量最高。该实验为东北浆果的综合利用提供理论依据。
[Key word]
[Abstract]
In order to promote the development and utilization of berry-related functional products in Northeast China, the contents of total phenols, total flavonoids and total anthocyanins in 9 kinds of berries widely cultivated and common in Northeast China were determined and analyzed. The types and contents of phenolic monomers in berries were determined by high performance liquid chromatography. Antioxidant activity and anti-inflammatory activity of berries were evaluated by measuring DPPH· scavenging ability, ABTS+? scavenging ability, hyaluronidase inhibiting ability and albumin denaturation inhibiting ability, and correlation analysis was conducted. The results showed that the contents of total phenols, total flavonoids and total anthocyanins in blue honeysuckle and lingonberry were higher, which were 2.83 and 2.42 g (GAE) /100g DW, 0.99 and 2.09 g (Rutin) /100g DW, 104.33 and 414.73 mg/100g DW, respectively. And they had strong antioxidant and anti-inflammatory activities. The correlation results showed that the total phenolic content contributed the most to the antioxidant and anti-inflammatory activities of the berries. 19 monomer phenols were identified and quantified in 9 berries. Chlorogenic acid, cryptochlorogenic acid, arbutin and baicalein were all detected in berries. Chlorogenic acid and cryptochlorogenic acid were the main phenolic monomers in most berries. Among them, the monomer phenol species in lingonberry and the monomer phenol content in blue honeysuckle were the highest. The experiment provided a theoretical basis for the comprehensive utilization of berries in Northeast China.
[中图分类号]
[基金项目]
国家重点研发计划项目(2022YFD1600500)、中央高校基本科研业务费专项(2572022DX05)