该研究采用响应面法对羊肚菌多酚的提取工艺进行了优化，对溶剂类型、乙醇浓度、料液比、超声时间、超声温度、超声功率等提取条件进行了单因素优化，并对影响较为显著的三个因素进行Box-Behnken设计，得出了最佳工艺参数：乙醇体积分数10%，料液比1:67（g/mL），超声时间90 min，超声温度33 ℃，超声功率300 W，此工艺条件下提取羊肚菌多酚含量为22.39 mg/g。根据响应面实验数据分析，模型较为显著（P<0.000 1），失拟项不显著（P>0.05），拟合程度高（96.2%），三因素对得率影响顺序为：超声温度>料液比>超声功率。同时对各提取条件下的羊肚菌多酚进行了HPLC检测，共检测出10种酚类物质，且各单因素条件下提取的10种酚类物质的含量曲线也与福林酚比色法测定数据基本一致。

The extraction process of Morchella polyphenols was optimized via response surface methodology. In addition, single-factor optimization of solvent type, ethanol concentration, solid-liquid ratio, ultrasonic time, ultrasonic temperature, and ultrasonic power were optimized. A three-factor and three-level Box-Behnken design was then implemented based on the factors of greater influence. The optimal process was verified as follows: ethanol concentration of 10%, liquid-to-material ratio of 1:67 (g/mL), ultrasonic extraction time of 90 min, ultrasonic extraction temperature of 33 ℃, and ultrasonic power of 300 W. The polyphenol content extracted from morels under these process conditions was 22.39 mg/g. According to the analysis of response surface experimental data, the model was significant (P<0.000 1), the lack of fit was not significant (P>0.05), and the fitting degree was high (96.2%). The order of the three major factors affecting extraction rate was as follows: ultrasonic temperature > material-to-liquid ratio > ultrasonic power. The polyphenol contents in Morchella esculenta obtained through various extraction conditions were analyzed via HPLC. Ten phenolic compounds were detected. The content curves of these ten phenolic compounds extracted under various single-factor conditions were basically consistent with the data determined via Folin-Ciocalteu colorimetry.

贵州省基础研究计划（重点项目）（黔科合基础[2020]1Z029）；贵阳学院硕士研究生科研创新计划项目（GYU-YJS[2021]-46）