该研究旨在探讨紫菜联合美多芭的抗帕金森（Parkinson’s Disease，PD）作用及其对肠道菌群的影响。以48只C57BL/6J小鼠为研究对象，随机分为4组：正常对照组、帕金森模型组、美多芭治疗组和紫菜联合美多芭治疗组。以添加5%（m/m）紫菜的饲料对PD小鼠干预2周后，进行行为学测试、免疫组化分析、免疫印迹分析、炎症因子测定；利用Illumina平台对小鼠肠道细菌的DNA片段进行双端测序。结果表明，相较于单独使用美多芭，紫菜联合美多芭治疗可以显著改善PD小鼠的脑功能，其中黑质中神经元的数量、酪氨酸羟化酶（TH）的表达量分别增加了46.95%、52.91%，α-突触核蛋白（α-syn）的表达量减少了34.56%；显著降低了IL-1β的含量，改善其炎症水平。16S rRNA结果表明，紫菜联合美多芭治疗组小鼠肠道菌群的丰富度提升、多样性增加；Firmicutes/ Bacteroidota的比值降低了71.59%；与抗炎水平相关的Muribaculaceae、Akkermansia菌属显著富集，而Blautia、Helicobacter等炎症相关菌属相对丰度明显下降。相较于单独服用美多芭，紫菜联合治疗后，其调节肠道菌群、减轻炎症的效果更佳，抗帕金森作用更显著。

This study aimed to investigate the anti-parkinsonism effect of laver combined with madopar and its influence on the intestinal flora of model mice. Forty-eight C57BL/6J mice were randomly divided into four groups: normal control, Parkinson’s disease (PD) model, madopar treatment, and laver combined with madopar treatment. Mice in the laver combined with madopar treatment group were fed a diet supplemented with 5% laver for 2 weeks. Subsequently, behavioral tests, immunohistochemistry, western blotting, and inflammatory factor level determination were performed to evaluate the brain function of the experimental mice. Paired-end sequencing of intestinal bacterial DNA was conducted on an Illumina platform to monitor changes in the intestinal flora. The results showed that, compared with madopar alone, laver combined with madopar significantly improved the brain function of PD model mice. The number of neurons and expression level of tyrosine hydroxylase in the substantia nigra were 46.95% and 52.91% higher, respectively, while the expression of α-synapse nucleoprotein was 34.56% lower. Serum IL-1β content was significantly lower, and inflammation was lower. Additionally, 16S rRNA sequencing showed that the richness and diversity of the intestinal flora were higher in the laver combined with madopar treatment group than in the madopar alone group. The ratio of Firmicutes/Bacteroidota was 71.59% lower. The relative abundance of Muribaculaceae and Akkermansia, which are related to anti-inflammation, were significantly enriched, whereas the relative abundances of Blautia, Helicobacter, and other inflammatory bacteria were significantly decreased. Compared with madopar alone, combined treatment with laver had more positive effects on the composition of the intestinal flora, reduced inflammation, and exerted stronger anti-parkinsonism effects.

山东省重点研发计划项目（2019GHY112058）