玉米赤霉烯酮（Zearalenone，ZEN）是广泛污染玉米、小麦、大米等谷物的真菌毒素之一，主要由镰刀菌属产生。该研究选择姜黄素为光敏剂构建光动力技术（Photodynamic Inactivation，PDI），探究姜黄素浓度、光照能量和溶液pH对PDI抑制禾谷镰刀菌生长及其在玉米中产ZEN的影响。同时，从ZEN毒素含量变化和禾谷镰刀菌失活两个角度分析PDI抑制禾谷镰刀菌产ZEN的机制。结果表明，在50.00 μmol/L姜黄素和43.0 J/cm2光照能量作用下，ZEN含量从空白对照玉米中的1.15 mg/kg降低至光动力处理组中的0.58 mg/kg，降幅达49.56%，且继续增加姜黄素浓度能够完全抑制ZEN的生成。深入分析表明，PDI对禾谷镰刀菌的灭活作用是其抑制玉米中ZEN产生的主要原因，尤其当姜黄素浓度≥70.00 μmol/L，光照能量为43.0 J/cm2时，禾谷镰刀菌被完全灭活。因此，该研究证实光动力技术能够有效地杀灭禾谷镰刀菌，抑制ZEN的产生，为保障农产品质量安全提供了一种新方法。

Zearalenone (ZEN) is a mycotoxin that commonly contaminates crops like maize, wheat, and rice, and is mainly produced by Fusarium graminearum. In this study, a photodynamic inactivation (PDI) technique was developed using curcumin as a photoactivator, and the effects of curcumin concentration, illumination energy, and solution pH on the PDI of F. graminearum growth and ZEN production in maize were investigated. Additionally, the mechanism by which PDI inhibits ZEN production by F. graminearum was analyzed in terms of ZEN levels and F. graminearum inactivation. The results demonstrated that 50.00 μmol/L of curcumin and 43.0 J/cm2 of illumination energy reduced ZEN levels in maize from 1.15 mg/kg in the control to 0.58 mg/kg in the treatment group (a decrease of 49.56%). Furthermore, ZEN production can be completely inhibited by increasing the curcumin concentration. Further mechanistic analysis revealed that PDI inhibits ZEN production in maize by inactivating F. graminearum. Notably, curcumin concentrations of ≥70.00 μmol/L with an illumination energy of 43.0 J/cm2 were sufficient to fully inactivate F. graminearum. By demonstrating that PDI is an effective method for F. graminearum inactivation and the inhibition of ZEN production, this work has provided a new method for quality and safety assurance in agricultural products.

国家自然科学基金项目（32102105）；广东省基础与应用基础联合基金项目（2020A1515110960）；佛山科学技术学院学生学术基金立项项目（xsjj202108zrb04）；佛山科学技术学院高层次人才启动项目（CGZ07001）；佛山科学技术学院学科建设项目（CGZ0400162）