该研究从水产市场污水中分离纯化副溶血性弧菌噬菌体，并对其中一株噬菌体vB_VpP_AC2（AC2）进行分离鉴定、基因组及部分生物学特性分析，探究该噬菌体作为副溶血性弧菌生防制剂的潜力。噬菌体AC2的噬菌斑透明且边缘清晰，透明部分的直径约为1.12 mm，无可见晕圈。噬菌体AC2的全基因组序列长44 270 bp，鸟嘌呤-胞嘧啶（GC）含量为49.30%，共预测到55个假定的开放阅读框，其中37个与编码已知功能蛋白质的基因相似（占67.27%），包括一个类似holin功能的蛋白（AC2_gp43，UTQ72417.1），该功能蛋白首次在Maculvirus属中被表征。经蛋白网络与末端酶系统发育树分析鉴定，噬菌体AC2属于自转录病毒科（Autograohiviridae），Maculvirus属，与噬菌体vB_VpaP_MGD1的ANIb值最高，为95.62%。在生物学特性方面，噬菌体AC2能够裂解30.77%的受测菌株，其最佳感染复数为0.001~0.1，一步生长曲线显示AC2的潜伏期为20 min，裂解期为35 min，裂解量为143 pfu/cell。总之，噬菌体AC2的分离鉴定不仅可为相关功能蛋白的研究提供参考，还可为水产食品安全提供较高应用价值的噬菌体资源。

Vibrio parahaemolyticus phages were isolated and purified from fish market effluent. One particular phage, phage vB_VpP_AC2 (AC2), was Vibrio parahaemolyticus phages were isolated and purified from fish market effluent. One particular phage, phage vB_VpP_AC2 (AC2), was isolated, genetically sequenced, and subjected to partial biological analysis to investigate its potential as a biocontrol agent against V. parahaemolyticus. The viral plaques of phage AC2 were transparent with clear boundaries; the diameter of the transparent parts was approximately 1.12 mm, with no visible halos. The genome sequence of phage AC2 was 44 270 bp long, with a guanine-cytosine content of 49.30%. In all, 55 putative open reading frames were predicted, and 37 (67.27%) of these were similar to genes encoding proteins of known function, including a holin-like protein (AC2_gp43, UTQ72417.1) that was first characterized in the genus Maculvirus. The results of protein-protein interaction network and terminase phylogenetic analyses showed that phage AC2 belongs to the family Autograohiviridae and genus Maculvirus. The Vibrio phage vB_VpaP_MGD1 shared the highest Average Nucleotide Identity based on BLAST (ANIb) value with AC2, at 95.62%. Biological analysis revealed that phage AC2 lysed 30.77% of the tested strains, with optimal multiplicities of infection of 0.001~0.1. Its one-step growth curve showed that its latent and lytic phases were 20 and 35 min long, respectively, with a burst size of 143 pfu/cell. In summary, the isolation and identification of phage AC2 provided useful information on its functional proteins, indicating that it is a valuable phage for aquatic food safety.

国家自然科学基金项目（32202194）；广东省基础与应用基础研究重大项目（2020B0301030005）