研究恭城油茶对D-半乳糖致衰老小鼠认知障碍的改善作用。将KM小鼠随机分为正常组、衰老模型组、Vc干预组（100 mg/kg）、恭城油茶干预组（750 mg/kg）。Morris水迷宫实验评价小鼠学习记忆能力；检测各组小鼠海马体和脑指数；比色法测定海马体、脑和肠道组织中谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）活性、总抗氧化能力（T-AOC）和丙二醛（MDA）水平；取部分海马体和脑组织制备切片，观察其病理变化。结果发现，恭城油茶能显著升高衰老小鼠海马体指数（35.56%）和脑指数（42.16%）（P<0.05）；增加平台象限游泳时间比例（20.44%）和平台象限游泳路程比例（16.10%）（P<0.05）；提高海马体中GSH-Px（37.95%）、T-SOD（86.83%）、T-AOC活性（199.22%）（P<0.05），降低MDA含量（65.66%）（P<0.05）；增加脑组织中GSH-Px（331.49%）和T-SOD活性（177.16%）（P<0.05）；切片结果显示，恭城油茶可缓解衰老过程中脑部的损伤。综上，恭城油茶可通过减轻氧化应激改善D-半乳糖引起的认知障碍。

The effect of Gongcheng oil tea (Camellia oleifera) on cognitive impairment in D-galactose-induced aging mice was investigated in this study. KM mice were randomly divided into four groups: normal group, aging model group, Vc intervention group (100 mg/kg), and C. oleifera intervention group (750 mg/kg). The Morris water maze test was used to analyze the learning and memory abilities of the mice, and their hippocampus and brain indices were determined. The activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC), as well as the malondialdehyde (MDA) level in the hippocampus, brain, and intestinal tissues of mice were determined through colorimetric analysis. Samples of hippocampus and brain tissue were extracted to prepare slices for the observation of pathological changes. The results showed that the hippocampus (35.56%) and brain indices (42.16%) in C. oleifera intervention group were significantly higher than those in the aging model group. Pre-treatment with C. oleifera significantly increased the proportion of swimming time and swimming distance in the platform quadrant (20.44% and 16.10%, respectively) (P<0.05), increased the activities of GSH-Px (37.95%), T-SOD (86.83%), and T-AOC (199.22%), and decreased MDA content (65.66%) in the hippocampus (P<0.05). The activity of GSH-Px (331.49%) and T-SOD (177.16%) in the brain tissues of the C. oleifera intervention group were higher than that in the aging model group (P<0.05). Analysis of the slices showed that C. oleifera could also relieve brain damage during aging. Thus, this study showed that C. oleifera can improve cognitive impairment caused by D-galactose, which may be associated with the attenuation of oxidative stress.

国家自然科学基金地区科学基金项目（82060099）；广西自然科学基金资助项目（2014GXNSFBA118124）；桂林医学院科研启动基金项目（04010140035）