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[摘要]
为开发黑参多糖在食品药品领域的应用潜力,以黑参多糖为原料利用高效液相色谱等方法测定多糖的理化性质,通过体外抗氧化和对癌细胞抑制率的实验评估多糖的抗氧化和抗肿瘤活性。结果表明:黑参多糖经SephadexG-200葡聚糖凝胶色谱柱分离出具有单一组分的BGP-1,其分子量约为1.80×106 u,主要由葡萄糖、阿拉伯糖和甘露糖组成的,摩尔比为70.52:17.35:5.63,同时还含有少量的半乳糖和木糖。紫外光谱扫描得出黑参多糖已无明显的杂质,傅里叶红外光谱显示其是含有α-糖苷构型的中性多糖。抗氧化实验中黑参多糖在0.8~4.0 mg/mL范围内具有一定的抗氧化活性,均可达到同浓度Vc作用下62%以上的效果。MTT法和流式细胞术实验证实黑参多糖对MGC-803胃癌细胞的生长进行抑制,在4.0 mg/mL抑制率达到85.30%,Western Blot结果发现0.8~4.0 mg/mL的黑参多糖通过线粒体途径的信号传递来促进细胞凋亡。综上证实黑参多糖具有抗氧化和抗肿瘤活性,可经研发后应用于药物制剂领域。
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[Abstract]
In order to develop the application potential of black ginseng polysaccharides in the food and medicine industries, the physical and chemical properties of polysaccharides were determined using high performance liquid chromatography and other methods. The antioxidant and antitumor activities of polysaccharides were evaluated through in vitro antioxidative and tumor cell inhibition experiments. A single component, BGP-1 (molecular weight 1.80×106 u) was separated from black ginseng polysaccharide using a Sephadex G-200 column chromatography. The polysaccharides were found to be mainly composed of glucose, arabinose, and mannose, with a molar ratio of 70.52:17.35:5.63, and also contained a small amount of galactose and xylose. Ultraviolet spectrum scanning showed that the black ginseng polysaccharide had no obvious impurities, and Fourier infrared spectrum showed that it was a neutral polysaccharide with α-glycoside configuration. In the antioxidant experiment, polysaccharides from black ginseng showed a certain antioxidant activity in the range of 0.8~4.0 mg/mL, and all could achieve the effect of more than 62% under the same concentration of Vc. The MTT method and flow cytometry experiments confirmed that black ginseng polysaccharides inhibited the growth of MGC-803 gastric cancer cells, and the inhibition rate reached 85.30% at 4.0 mg/mL. The Western Blot results showed that 0.8~4.0 mg/mL black ginseng polysaccharide promoted cell apoptosis through the signal transduction of the mitochondrial pathway. In conclusion, black ginseng polysaccharides have antioxidant and antitumor activities, which can be used in the field of pharmaceutical preparations after research and development.
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