该研究旨在初步考察复合红花籽油的肠吸收特性。采用外翻肠囊法对其肠吸收部位、时间、给药浓度进行研究，通过气相色谱-质谱法（GC-MS）测定肠收集液中亚油酸（Linoleic Acid，LA）及α-亚麻酸（α-Linolenic Acid，ALA）含量，通过计算吸收转运参数、吸收速率参数分析其肠道转运机制。不同肠段中，其主要成分LA与ALA均能在十二指肠（LA：2.71 mg；ALA：1.60 mg）与空肠（LA：1.89 mg；ALA：1.43 mg）中被检出，通过计算不同部位透过药量（M）、药物吸收转化率（A）和药物剩余百分数（R），结果显示十二指肠段中LA、ALA的M高于空肠段，A与空肠段接近，R低于空肠段，因此十二指肠为最佳吸收肠段。在120 min时，LA和ALA的Pappi（瞬时表观渗透系数）皆高于其他时间点，因此最佳吸收转运时间为120 min。在给药倍数为2.0倍时，肠囊液中的LA和ALA浓度最高，分别为0.35、0.19 mg/mL，因此最佳给药浓度为200 mg/mL。结果表明复合红花籽油的肠吸收特性可能为具有时间与浓度依赖性的被动运输模式且在十二指肠段中单向转运。

The intestinal absorption characteristics of compound safflower seed oil was investigated. Its intestinal absorption site, time and loading concentration were investigated by the everted gut sac technique. The contents of linoleic acid (LA) and α-linolenic acid (ALA) in the the collected intestinal fluid were determined by gas chromatography-mass spectrometry (GC-MS). The intestinal transport mechanism was analyzed through calculating the absorption transport parameters and absorption rate. In different intestinal segments, the main components LA and ALA were detected in both the duodenum (LA: 2.71 mg; ALA: 1.60 mg) and jejunum (LA: 1.89 mg; ALA: 1.43 mg). By calculating the permeation volume (M), absorption conversion rate (A) and residue percentage (R) at different sites, it was found that the M values of LA and ALA in the duodenum were higher than those for the jejunum, the A values were close to those for the jejunum, and the R values were lower than those for the jejunum. Therefore, the duodenum was the best absorption site among the intestinal segments. At the 120th min, the Pappi (apparent permeability coefficient) values of LA and ALA were higher than those of other time points, thus, the optimal absorption and transport time was 120 min. When the loading dose was 2.0 times, the concentrations of LA and ALA in the intestinal sac fluid were the highest (0.35 and 0.19 mg/mL, respectively), thus, the optimal loading dose was 200 mg/mL. The results showed that the intestinal absorption of the compound safflower seed oil may take place in a time- and concentration-dependent passive transport mode and via one-way transport in the duodenum.

国家自然科学基金资助项目（31960475）