该研究以不老莓多肽粉为研究对象，研究其体外抗氧化功能以及对小鼠免疫功能的影响。通过测定小鼠体内脾淋巴细胞转化、迟发型变态反应、血清溶血素等指标，结果发现高剂量组与阴性对照组相比，可以明显提高小鼠的迟发型变态反应（P=0.009）、抗体生成能力（P=0.046）、血清溶血素水平（P=0.040）、碳廓清能力（P=0.001）和NK细胞活性（P=0.018）；中剂量组对小鼠脾淋巴细胞增殖能力有显著增强作用（P=0.038）；低剂量组具有增强小鼠碳廓清能力（P=0.042）；其次通过对DPPH自由基测定、羟自由基和超氧阴离子自由基试剂盒的分析，结果显示半清除浓度IC50分别为0.07、13.91、10.00 mg/mL，总抗氧化能力为72.76 U/mL，且DPPH自由基、羟自由基、超氧阴离子自由基的清除率均与不老莓多肽粉的质量浓度呈量效关系。上述研究结果明确了不老莓多肽粉不仅具有增强小鼠机体免疫功能的作用，而且具有一定的抗氧化功能。

In this study, the polypeptide powder of Aronia melanocarpa was taken as the research object for investigating its in vitro antioxidant function and its effect on the immune function of mice. By measuring the transformation of spleen lymphocytes, delayed-type hypersensitivity, serum hemolysin and other indicators of mice, it was found that compared with the negative control group, the high-dose group had significantly increased delayed-type hypersensitivity (P=0.009), antibody-producing ability (P=0.046), serum hemolysin level (P=0.040), carbon clearance ability (P=0.001) and NK cell activity (P=0.018); the medium-dose significantly enhanced the proliferation ability of spleen lymphocytes in mice (P=0.038); the low-dose could enhance the carbon clearance ability in mice (P=0.042); Secondly, through the analyses using the DPPH free radical assay, and hydroxyl radical and superoxide anion radical kits, it was found that the half-scavenging concentrations, IC50 values were 0.07 mg/mL, 13.91 mg/mL, and 10.00 mg/mL, respectively, and the total antioxidant capacity was 72.76 U/mL. The scavenging rates for DPPH free radicals, hydroxyl radicals, and superoxide anion free radicals were all dose-dependent with the concentration of berry polypeptide. The research results showed that the polypeptide powder of Aronia melanocarpa not only can enhance the immune function

河南省重点研发与推广专项（科技攻关）项目（202102110130；192102110075）；河南省重大科技专项（181100211400）；河南牧业经济学院重点学科项目（C3060020）