该研究探究了姜黄素介导的光动力技术对扩展青霉菌丝生长及分泌棒曲霉素的影响。结果表明，固体培养扩展青霉菌丝5 d后活力旺盛，棒曲霉素分泌量增长6.5倍。仅姜黄素短暂处理可轻微抑制菌丝生长及棒曲霉素分泌；而在0.275 W/cm2光剂量下，50~300 μmol/L浓度的姜黄素可显著降低固体培养扩展青霉的菌落直径（0.84 cm）和菌丝质量（68.37 mg），同时棒曲霉素分泌抑制率约为83.85%。接种菌丝的红富士苹果经光动力处理并培养8 d后，病斑直径抑制率与姜黄素浓度呈正相关性，最大抑制率为49.38%；而棒曲霉素在果实内分布与菌丝扩展能力密切相关，病斑比重和棒曲霉素含量分别为0.09%、18.02%。综上，姜黄素介导的光动力技术可显著抑制扩展青霉菌丝生长以及棒曲霉素分泌。

The effects of curcumin-mediated photodynamic technology (CUR-PDT) on the mycelial growth and patulin (PAT) secretion of Penicillium expansum were investigated. Mycelia of P. expansum grew vigorously after 5 days of solid culture, and PAT secretion increased 6.5 times. Short-term treatment with curcumin slightly inhibited the mycelial growth and PAT secretion. At a low dose of 0.275 W/cm2, 50~300 μmol/L curcumin significantly reduced the colony diameter (0.84 cm) and mycelial weight (68.37 mg) of P. expansum in solid culture, and the inhibition rate of PAT secretion was 83.85%. Following the inoculation of Red Fuji apples with PDT-treated mycelia and culture for 8 days, the inhibition rate of lesion diameter was positively correlated with the concentration of curcumin, with the maximum inhibition rate of 49.38%. The distribution of PAT in the fruit was closely correlated with the mycelial expansion ability, and the lesion proportion and PAT content were 0.09% and 18.02% respectively. Therefore, CUR-PDT can significantly inhibit the mycelial growth and PAT secretion of P. expansum.

国家自然科学基金项目（32172275）；山东省自然科学基金项目（ZR2019BC049）