研究不同枸杞（Lyciumbarbarum，Gouqi，GQ）多糖组分对苯甲酸雌二醇（Estradiol，E2）及黄体酮（Progesterone，P）联合诱导小鼠乳腺上皮细胞（HC11细胞）增殖的抑制作用的影响。通过水提醇沉及柱分离法获得不同组分；并对其分子量及体外抗增殖、抗炎症作用进行初步分析。结果显示，枸杞粗多糖通过DEAE-52离子色谱柱和Sephadex G-50凝胶柱洗脱出7种多糖组分，分别为GQ-01、GQ-02、GQ-03、GQ-04、GQ-05、GQ-06、GQ-07，其平均相对分子量分别为113 ku、<1 ku、367 ku、613 ku、81 ku、110 ku、<1 ku。选用不同浓度枸杞多糖（5、25、125、250、500、1 000 μg/mL）处理HC11细胞，发现250 μg/mL（GQ-01、GQ-04及GQ-06）可显著抑制E2（25 μg/mL）+P（250 μg/mL）联合诱导的HC11细胞增殖活性，分别降低了28.13%、26.74%、30.83%（P<0.01）；GQ-01及GQ-06可调节G0/G1与G2/M期转变而影响细胞周期的分布；与模型组相比，GQ-06可极显著抑制E2+P联合诱导雌激素受体（ERα）水平，降低了5.54%（P<0.01）；GQ-04及GQ-06可显著抑制LPS诱导RAW264.7细胞的NO水平的产生，与模型组相比NO水平分别降低了13.43%、12.65%（P<0.05）。该研究表明，GQ-06可能通过影响细胞周期分布及抑制ERα的水平，从而抑制乳腺上皮细胞过度增殖；此外，还可抑制LPS诱导RAW364.7细胞中NO的释放，降低细胞的炎症反应。该研究将为功能性食品深度开发提供重要的理论依据。

To investigate the effects of Lycium barbarum polysaccharides on the proliferation of Estradiol (E2) and progesterone (P) induced-mouse mammary gland epithelial cells (HC11) and LPS-induced inflammatory response in macrophages. Different components were obtained by water extraction, ethanol precipitation and column separation, and their molecular weights, and in vitro anti-proliferative and anti-inflammatory effects were preliminarily analyzed. The result showed that seven Lycium barbarum polysaccharides components were eluted out by DEAE-52-ion chromatography column and Sephadex G-50 gel column (GQ-01, GQ-02, GQ-03, GQ-04, GQ-05, GQ-06 and GQ-07, respectively), and their mean relative molecular weights were 113 ku, <1 ku, 367 ku, 613 ku, 81 ku, 110 ku, and <1 ku. Different Lycium barbarum polysaccharide concentrations (5, 25, 125, 250, 500, 1 000 μg/mL) were used to treat HC11 cells, and GQ-01, GQ-04 or Gq-06 at 250 μg/mL could significantly inhibit the proliferation of HC11 cells co-treated with E2 (25 μg/mL) and P (250 μg/mL) by 28.13%, 26.74% and 30.83%, respectively (P<0.01). GQ-01 and Gq-06 could regulate the G0/G1 and G2/M phase transition to affect the cell cycle distribution. Compared with the model group, Gq-06 could significantly inhibit the E2+P induced ERα level (decreased by 5.54%) (P<0.01). Compared with the model group, the production of NO in macrophages could be significantly inhibited by GQ-04 and GQ-06 (by 13.43% and 12.65%, respectively) (P<0.05). This research showed that GQ-06 may inhibit the excessive proliferation of mammary gland epithelial cells by affecting the cell cycle distribution and the level of ERα, and also suppress LPS-induced NO release to reduce the inflammatory response in RAW264.7 cells. This study will provide an important theoretical basis for further development of functional foods.

国家自然科学基金项目（22178070）；科技兴蒙项目（2021CG0029）