该研究旨在探讨药食同源组方联合益生菌对环磷酰胺（CTX）诱导免疫低下小鼠免疫功能的调节作用。除空白对照组用生理盐水灌胃外，其余各组小鼠腹腔注射环磷酰胺（CTX）100 mg/kg（按小鼠体质量计），连续腹腔注射4 d。将建模成功体重相近的小鼠随机分为6组：正常对照组、免疫抑制组、药食同源组方Ⅲ号、药食同源改良组（Ⅳ号）、益生菌组（Ⅴ号）和Ⅳ和Ⅴ联用组（Ⅵ号）。连续灌胃给药14 d，观察Ⅲ、Ⅳ、Ⅴ和Ⅵ对小鼠胸腺指数、脾脏指数、吞噬能力、脾淋巴细胞增殖、NK细胞活性以及血清中IgG、IgA和IgM生成水平的影响。结果显示：Ⅲ、Ⅳ、Ⅴ和Ⅵ组均能显著提高模型小鼠胸腺指数（1.46、1.67、1.50、1.92 g/kg）、脾脏指数（2.32、2.54、2.52、2.82 g/kg）、吞噬能力（0.43、0.45、0.44、0.62）、脾淋巴细胞增殖作用（1.39、1.45、1.42、1.43）、NK细胞活性（0.35、0.43、0.36、0.71）（P<0.05）；Ⅳ、Ⅴ和Ⅵ均能促使IgG（224.21、163.21、253.35 mg/L）、IgA（34.86、22.09、41.17 mg/L）和IgM（84.01、78.23、100.28 mg/L）生成水平均明显提高（P<0.05）。研究结果表明，药食同源改良组方与益生菌联用组对环磷酰胺致免疫低下小鼠的免疫能力具有显著增强作用。

The aim of this study was to investigate the regulatory effect of the combination of drug-food homologous prescription and probiotics on the immune function of immunocompromised mice induced by cyclophosphamide (CTX). Except for the control group gavaged with normal saline, the mice in the other groups were intraperitoneally injected with CTX (100 mg/kg bw, for 4 days). Mice with similar body weights were randomly divided into 6 groups: control group, immunosuppression (model) group, drug-food homologous group III, drug-food homologous improvement group (IV), probiotics group (V), and IV-V combination group (VI). After the administration for 14 days, the effects of III, IV, V and VI on the thymus and spleen indices, phagocytic ability, spleen lymphocyte proliferation, NK cell activity and the levels of the formed IgG, IgA and IgM in immunodepressed mice were assessed. The results showed that the thymus index (1.46, 1.67, 1.50 and 1.92 g/kg) and spleen index (2.32, 2.54, 2.52 and 2.82 g/kg), phagocytic ability (1.39, 1.45, 1.42 and 1.43), spleen lymphocyte proliferation (1.39, 1.45, 1.42 and 1.43) and NK cells activity (0.35, 0.43, 0.36 and 0.71) were enhanced significantly (P<0.05) in the group III, IV, V and VI. IV, V and VI all significantly increased the levels of IgG (224.21, 163.21 and 253.35 mg/L), Ig A (34.86, 22.09 and 41.17 mg/L), and IgM (84.01, 78.23 and 100.28 mg/L) (P<0.05). These results suggested that the combination of the modified drug-food homologous prescription and probiotics could enhanced significantly the immune function of the CTX-immunosuppressed mice.

国家自然科学基金项目（81502947）；天津市教委基金项目（20140219）；龙口南山养生谷肿瘤医院项目（20210906）