玉米须总黄酮含量检测络合显色体系的比较

Comparison of Complexation Color System for Detection of Total Flavonoids Content in Corn Silk

摘要:

采用紫外可见分光光度法建立最适合玉米须总黄酮(Corn Silk Total Flavonoids，CSTF)含量检测的络合显色体系。实验选用体积分数60%乙醇为溶剂对CSTF进行提取，并以不同极性溶剂对CSTF进行分级萃取，最终获得正己烷(Normal Hexane，n-Hexane)、乙酸乙酯(Ethyl Acetate Extracts，EAE)和正丁醇(Bormal Butanol，n-BuOH)萃取物。以玉米须中不同类型黄酮为对照品，比较研究NaNO2-Al(NO3)3/AlCl3-NaOH和KAc-Al(NO3)3/AlCl3 四种络合显色体系下乙酸乙酯和正丁醇萃取物中总黄酮含量。同时采用高效液相色谱(High Performance Liquid Chromatography，HPLC)对乙酸乙酯和正丁醇萃取物中黄酮含量进行测定，从而确定最适合的对照品种类和显色体系。结果表明，HPLC法检测乙酸乙酯和正丁醇中黄酮含量分别为83.45%和57.36%，经比较研究发现，与HPLC法检测总黄酮含量最接近的是以芦丁为标准品的KAc-Al(NO3)3络合显色体系。综上所述，KAc-Al(NO3)3络合显色体系是玉米须和各萃取物总黄酮含量检测的最佳方法，该方法检测CSTF提取液黄酮含量为1.08%。该方法操作简便、准确度高，可为玉米须黄酮类物质的开发利用和质量管控提供依据。

Abstract:

UV-visible spectrophotometry was used to establish the most suitable complex chromogenic system for the detection of Corn Silk Total Flavonoids (CSTF) content. In the experiment, 60% ethanol was used as solvent to extract CSTF, and CSTF was extracted by different polar solvents. Finally, n-Hexane, ethyl acetate and n-Butanol extracts were obtained. The contents of total flavonoids in ethyl acetate and n-Butanol extracts under NaNO2-Al(NO3) 3/AlCl3-NaOH and KAc-Al(NO3)3/AlCl3 complexation systems were compared with different types of flavonoids in corn silk. The content of flavonoids in ethyl acetate and n-Butanol extracts was determined by High Performance Liquid Chromatography (HPLC) to identify the most suitable reference standard and color system. The results showed that the contents of flavonoids in ethyl acetate and n-Butanol were 83.45% and 57.36%, respectively. The results showed that the KAc-Al(NO3)3 complex color system with rutin as the reference standard was the closest to the content of total flavonoids detected by HPLC. In summary, the KAc-Al(NO3)3 complex chromogenic system is the best method for detecting the total flavonoid content of corn silk and each extract. This method detects the flavonoid content of CSTF extract is 1.08%. The method is simple and accurate, which can provide a basis for the development and utilization of flavonoids in corn silk and quality control.