为充分利用鲳鱼资源及实现虾青素的稳态化递送，本实验通过等电点沉淀法提取得到银鲳蛋白，基于静电相互作用制备了银鲳蛋白/壳聚糖复合颗粒来改善鱼肉蛋白乳化性，探究乳液的稳定性及对虾青素的递送能力。结果表明，壳聚糖显著提高了银鲳蛋白的疏水性，复合颗粒水接触角达到84.10°，具有更好的乳化活性和乳化稳定性，其制备的乳液在储存14 d后仍可保持稳定。体外模拟消化结果表明，银鲳蛋白/壳聚糖复合颗粒乳液的游离脂肪酸释放率和虾青素生物可及性最高，分别达到38.53%和36.05%，相较于银鲳蛋白乳液，促进了脂质的消化和虾青素的递送，证实银鲳蛋白/壳聚糖复合颗粒稳定的乳液是一种优异的功能因子递送载体。本研究为鲳鱼资源的高值化利用及虾青素的高效递送提供实验依据，有利于促进海洋生物资源的充分利用。

In order to fully utilize pomfret resources and achieve the steady delivery of astaxanthin, Pampus argenteus protein was extracted via isoelectric precipitation. Furthermore, Pampus argenteus protein/chitosan composite particles were prepared by electrostatic interaction to improve the emulsifying property of fish protein. The stability and astaxanthin delivery capacity of this emulsion were investigated. The results show that the hydrophobicity of Pampus argenteus protein was significantly improved by chitosan. The water contact angle of composite particles reached 84.10°, and the composite particles had better emulsifying activity and stability. The emulsion fabricated using the composite particles remained stable after 14 days of storage. In vitro simulated digestion showed that the free fatty acid release rate and astaxanthin bioaccessibility of the emulsion stabilized by Pampus argenteus protein/chitosan composite particles were the highest, reaching 38.53% and 36.05%, respectively. Compared with the emulsion stabilized by Pampus argenteus protein, the emulsion stabilized by Pampus argenteus protein/chitosan composite particles promoted lipid digestion and astaxanthin delivery. This shows that the emulsion stabilized by Pampus argenteus protein/chitosan composite particles was an excellent delivery system for functional factors. This study provides an experimental basis for the high-value utilization of pomfret resources and highly efficient transport of astaxanthin, which is beneficial for making full use of marine biological resources.

山东省优秀青年科学基金项目（2022HWYQ-068）；中国海洋大学“青年英才工程”科研启动项目(862001013134)