建立同步测定香蕉全果、香蕉果肉和香蕉叶中烯效唑、多效唑等9种植物生长调节剂（Plant Growth Regulators，PGRs）的多功能过滤器净化-超高效液相色谱-串联质谱法（UPLC-MS/MS），为香蕉中多种PGRs残留的同步测定提供技术参考。样品经1% （V/V）乙酸-乙腈提取，多功能针式过滤器净化，净化后的提取液以UPLC-MS/MS在选择反应监测模式下对烯效唑等9种PGRs目标物残留进行测定。在0.001~0.50 mg/L范围内，9种PGRs的浓度与峰面积呈良好的线性关系，相关系数均大于0.9992。在0.05、0.10、0.50 mg/kg的加标浓度下，回收率在80.50%~113.00%之间，相对标准偏差（RSD）在0.40%~5.60%之间，检出限（LOD）在0.20~10.00 μg/kg之间，定量限（LOQ）在0.70~33.50 μg/kg之间。该方法快速简便、灵敏准确，可用于同步测定烯效唑等9种PGRs在香蕉全果、香蕉果肉及香蕉叶中的残留。

A method was developed for the simultaneous determination of nine plant growth regulator (PGR) residues, such as uniconazole and paclobutrazol, in whole banana fruit, banana pulp, and banana leaves by combining ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and multifunctional needle filter methodology, to provide a reference for the simultaneous determination of multiple PGR residues in bananas. The analytes were extracted from a sample with 1% acetonitrile acetate, and purified by a multifunctional needle filter; the purified sample solutions were analyzed through UPLC-MS/MS in the selective reaction monitoring (SRM) mode for the detection of uniconazole and other PGR residues in the samples. The concentrations of nine PGRs showed good linearity with the corresponding peak area in the 0.001~0.50 mg/L range, and the correlation coefficient (r2) was more than 0.9992. The average recoveries at the spiked levels of 0.05, 0.10 and 0.50 mg/kg for all target compounds in the samples were 80.50%~113.00%, with relative standard deviations (RSD) between 0.40% and 5.60%. The limits of detection (LODs, S/N = 3) for the nine PGRs ranged from 0.20 to 10.00 μg/kg, and the limits of quantification (LOQs, S/N=3), (S/N=10) ranged from 0.70 to 33.50 μg/kg. The proposed method is quick, easy, sensitive and accurate, and can be used for the simultaneous determination of the above-mentioned nine PGR residues in whole bananas, banana pulp, and banana leaves.

广西省创新驱动发展专项资金项目（桂科AA20302016-6）；广西省自然科学基金面上项目（2020GXNSFAA159073）；广西农业科学院基本科研业务专项项目（桂农科2021YT139；2021YT138）；广西农业科学院科技发展基金项目（桂农科2021JM119）；科技先锋队“强农富民”“六个一”专项行动（桂科盟202216）