该研究以红参、燕窝、熟酸枣仁为主要原料研制出的红参-燕窝-酸枣仁复合助眠饮料最佳配方，并用其对Balb-c雄性小鼠连续灌胃30 d，通过直接睡眠、延长戊巴比妥钠睡眠时间、戊巴比妥钠阈下剂量睡眠、巴比妥钠睡眠潜伏期实验，及测定小鼠脑组织中相关指标，来讨论该复合饮品对睡眠的改善作用。结果显示，红参-燕窝-酸枣仁复合助眠饮料（低、中、高剂量）能延长戊巴比妥钠作用下小鼠的睡眠时间，分别延长了24.72%、66.11%、85.12%，中、高剂量增加戊巴比妥钠阈下剂量作用下小鼠的入睡率达到80%，同时可有效缩短巴比妥钠催眠下小鼠的睡眠潜伏期。此外，相比空白组、剂量组小鼠脑组织中5-HT含量显著升高，分别提高了398.28、742.34、830.08 ng/mL。同时中剂量组和高剂量小鼠脑组织中TPH含量显著升高，分别提高433.82、663.74 pg/mL。中、高剂量组小鼠脑组织中GABA含量显著升高，分别提高了0.04、0.06 μmol/L，同时Glu含量也显著增加，分别提高了0.20、0.50 μmol/L。综上，红参-燕窝-酸枣仁复合助眠饮料具有改善睡眠的作用，这一作用途径与通过调节脑组织中血清素系统与神经递质有关。

In the currently study, the optimal formula of a "red ginseng-bird's nest-jujube seed compound sleep aid drink" was developed using red ginseng, bird's nest and cooked sour jujube seeds as the main raw materials. The effect of the compound drink on sleep improvement was determined in the Balb-c male mice subjected to 30-day continuous oral gavage of the compound drink by the direct sleep test, prolonged pentobarbital sodium sleep time test, pentobarbital sodium subthreshold dose hypnotic test, and barbital sodium sleep latency test. The related indicators of mouse’s brain tissues were measured. With the results showed that red ginseng-bird's nest-jujube seed compound sleep aid drink at low, medium and high doses could prolong sleep time of the mice under the action of pentobarbital sodium by 24.72%, 66.11%, and 85.12%, respectively. The compound drink at the medium and high doses could also increase the sleep-onset rate to 80% in the mice treated with pentobarbital sodium at the subthreshold dose, while shortening effectively the sleep latency for the mice barbital sodium hypnosis. In addition, compared with the blank group, the 5-HT contents of the treatment groups increased significantly (increased by 398.28, 742.34, and 830.08 ng/mL for the low, medium and high dosage groups, respectively). The TPH contents of the medium and high dosage groups increased significantly (increased by 433.82 and 663.74 pg/, respectively). The GABA contents in the brain tissues of the mice in the medium and high dosage groups increased significantly (increased by 0.04 and 0.06 µmol/L, respectively), while the Glu contents of these two groups of mice also increased significantly (increased by 0.20 and 0.50 µmol/L, respectively). In summary, the red ginseng-bird's nest- jujube seed compound sleep aid drink can improve sleep, and such an effect is related to the regulation of the serotonin system and neurotransmitters in the brain tissue.

福建省科技创新领军人才项目（KRC16002A）；红参燕窝静心饮改善睡眠研究（KH210071A）