为探究枸杞鲜果保鲜机理，利用体积分数75%乙醇溶液、乳酸涂膜、壳聚糖涂膜和气调处理对枸杞鲜果进行保鲜处理，测定保鲜前后营养成分变化指标及相关酶活性，利用实时定量PCR（RT-qPCR）技术，测定枸杞抗坏血酸过氧化物（LcAPX）基因的相对表达量，同时测定枸杞鲜果贮存后的激素含量，研究不同处理方法对新鲜枸杞的保鲜效果及其对关键基因相对表达量的影响。结果表明，不同处理能明显提高保鲜效果，其中化学保鲜剂以脱乙酰度96.1%、浓度为1.5 g/L的壳聚糖溶液涂膜处理效果最佳，气调处理能保持枸杞鲜果的感官品质，降低相关酶的活性；同时，经保鲜处理的枸杞鲜果LcAPX基因相对表达量均低于1，气调处理效果最佳，相对表达量为0.35。贮存后的枸杞鲜果其生长素（Auxin/ICAld）上升幅度最大，由2.43上升至28.71，脱落酸（ABA）含量由3.59上升至18.10，均较空白对照组上升，乙烯（ETH）由17.70下降至8.56，赤霉素（GA）含量由17.7降低至6.49，与激素变化规律基本一致。

In order to explore the mechanism of fresh Lycium chinense fruit preservation, fresh Lycium chinense fruits were subjected to preservation treatments, 75% etohanol solution, lactic acid coating, chitosan coating and modified atmosphere treatment. The changes of nutrients and the activities of related enzymes before and after preservation were determined, Real-time quantitative PCR (qRT-qPCR) technology was used to determine the relative gene expression of ascorbate peroxide (LcAPX) in Lycium chinense, while the hormone content of fresh Lycium chinense fruit after storage was determined. The effects of different treatments on the preservation of fresh Lycium chinense and the relative expressions of key genes were studied. The results showed that different treatments could significantly improve the fresh-keeping effect. Among them, the best preservation was with the chemical, the chitosan coating solution with a degree of deacetylation of 96.1% and a concentration of 1.5 g/L. The modified atmosphere treatment could maintain the sensory quality of fresh fruits and reduce the activities of related enzymes. In the meantime, the relative expression levels of LcAPX gene in the fresh Lycium chinense fruits subjected to preservation treatments were all lower than 1, with that being 0.35 (the best) for the modified atmosphere treatment. After storage, the content of auxin (ICAld) of the fresh fruit exhibited the largest increase (increased from 2.43 to 28.71), while the content of ABA increased from 3.59 to 18.10, which were all higher than those of the blank control group. The content of ethylene (ETH) decreased from 17.70 to 8.56, and the content of gibberellin (GA) decreased from 17.7 to 6.49, which were essentially consistent with the changing trends of hormones.