该研究选取甘薯作为原料，利用米曲霉发酵制取甘薯去氢表雄酮（DHEA），探讨发酵后甘薯DHEA提取物纯化前后体外抗氧化、抗衰老、降血糖及抗癌功效。结果表明：甘薯DHEA提取物经硅胶吸附纯化时，样品保留时间为11.21 min，峰面积占比达76.44%。随着浓度上升，纯化前后甘薯DHEA提取物对DPPH、ABTS、羟基自由基的清除力、总还原能力呈显著上升趋势（p<0.05），在0.10 mg/mL时达到最大。纯化后DHEA提取物的α-淀粉酶活性抑制率在2.16%~55.54%之间，在0.80 mg/mL时达到最大；酪氨酸酶活性抑制率在5.55%~50.57%之间，在0.10 mg/mL时达到最大。纯化后的DHEA提取物仅对乳腺癌细胞MCF-7在2.30 mmol/L浓度水平时有显著的抑制效果（p<0.05），抑制率达47.50%，而对肺癌细胞A549、宫颈癌细胞Hela无显著的抑制效果（p>0.05）。该研究为甘薯DHEA的功能性食品的开发提供一定参考依据，有利于甘薯天然产物的开发。

In this study, sweet potato was selected as the raw material, dehydroepiandrosterone (DHEA) was prepared by Aspergillus oryzae fermentation. The in vitro antioxidant, anti-aging, hypoglycemic and anticancer effects of the DHEA extract from the fermented sweet potato before and after purification were examined. The results showed that the retention time was 11.21 min and the peak area was accounted for 76.44% when the sweet potato DHEA extract was purified by silica gel adsorption. With the increase of concentration, the scavenging abilities for DPPH, ABTS and hydroxyl radicals and the total reducing ability of the DHEA extract before and after purification increased significantly (p<0.05), and reached the maximum at 0.10 mg/mL. The α-amylase activity inhibitory rates of the purified DHEA extract ranged from 2.16% to 55.54%, and reached the maximum at 0.80 mg/mL. The tyrosinase activity inhibitory rates of the purified DHEA extract were in the range of 5.55%~50.57%, and reached the maximum at 0.10 mg/mL. The purified DHEA extract only exhibited a significant inhibitory effect against the breast cancer cell MCF-7 at a concentration of 2.30 mmol/L (p<0.05), with the inhibition rate being 47.50%. But the purified DHEA extract had insignificant effects against lung cancer cell A549 and cervical cancer cell Hela (p>0.05). This study provides a reference for the development of functional foods using sweet potato DHEA, which is conducive to the development of sweet potato natural products.

湖北省技术创新重大专项（2019AEE022）