为明确芡实壳醇提物对SGC7901及HepG2细胞体外抑制作用及机制。该研究采用75%乙醇对芡实壳超声提取，采用福林酚法测定醇提物总酚含量，采用CCK-8法检测醇提物对SGC7901和HepG2细胞的增殖作用抑制率并计算IC50值，使用流式细胞仪检测细胞凋亡、细胞周期、细胞线粒体膜电位及胞内钙离子浓度。结果表明，醇提物对SGC7901和HePG2细胞具有增殖抑制作用，浓度为200 μg/mL的醇提物作用细胞48 h后，抑制率分别为92.63%和72.40%。细胞周期检测表明，浓度为200~800 μg/mL的醇提物可使SGC7901细胞阻滞于G0/G1期；浓度为50~200 μg/mL的醇提物可使HepG2细胞阻滞于S期。细胞线粒体膜电位测定表明，醇提物可使SGC7901和HePG2细胞线粒体膜电位下降且具浓度依赖性，醇提物浓度为200 μg/mL时，细胞线粒体膜电位相较对照组分别下降21.92%和53.81%。细胞钙离子浓度测定表明，醇提物可使SGC7901和HePG2细胞内钙离子浓度升高且具浓度依赖性，醇提物浓度为200 μg/mL时，胞内钙离子浓度相较对照组分别上升21.85%和14.14%。实验表明芡实壳醇提物可抑制SGC7901和HepG2细胞增殖并诱导其凋亡，其作用机制可能与细胞线粒体膜电位降低及胞内钙离子浓度升高有关。

To clarify the in vitro inhibitory effect and mechanism of the alcoholic extract from Euryale ferox seed shell on human stomach cancer SGC7901 and human liver cancer HepG2 cells. In this study, 75% ethanol was used for ultrasonic extraction of Euryale ferox seed shell and the Folin-Phenol method was used to determine the content of total phenolics; the cell counting Kit-8 (CCK-8) was used to detect the inhibitory rates of the alcoholic extract on the proliferation of SGC7901 and HepG2 cells, and then the IC50 values were calculated. The flow cytometer was used to detect the apoptosis, cell cycle, cell mitochondrial membrane potential and intracellular calcium concentration. The results showed that the alcoholic extract had an inhibitory effect on the proliferation of SGC7901 cells and HepG2 cells, with the inhibition rate being 92.63% and 72.40%, respectively after the treatment with the extract at 200 μg/mL for 48 h. The cell cycle detection showed that the alcoholic extract at 200~800 μg/mL could arrest SGC7901 cells in the G0/G1 phase; the alcoholic extract at 50~200 μg/mL could arrest HepG2 cells in the S phase. The measurements of the cell mitochondrial membrane potential showed that the alcoholic extract could decrease the cell mitochondrial membrane potential of SGC7901 cells and HepG2 cells in a concentration-dependent manner, the cell mitochondrial membrane potential values of SGC7901 cells and HepG2 cells were reduced by 21.92% and 53.81%, respectively, compared with the control group, when the concentration of the alcoholic extract was at 200 μg/mL. The determination of intracellular calcium concentration showed that the alcoholic extract could increase the intracellular calcium ion concentration of SGC7901 cells and HepG2 cells in a concentration-dependent manner. When the extract concentration was 200 μg/mL, the intracellular calcium ion concentrations of SGC7901 cells and HepG2 cells increased by 21.85% and 14.14%, respectively, compared with the control group. Experimental results showed that the alcoholic extract of Euryale ferox seed shell could inhibit the proliferation and induce the apoptosis of SGC7901 and HepG2 cells, and the underlying mechanism might be related to the reduction of cell mitochondrial membrane potential and the increase of intracellular calcium ion concentration.

湖北省重点研发计划项目（2020BBB074）；武汉轻工大学校立重点项目（2013d11）