该研究以5 dpf斑马鱼为对象，系统研究了其对葡萄糖（GLU）和四氧嘧啶（ALX）耐受性的影响，研究了GLU与ALX联合建立糖尿病斑马鱼生物模型的可行性和有效性。以此为基础，研究了斑马鱼对青钱柳（CP）的耐受性并指导开展了CP的降糖作用评价。结果发现，以10 mg/mL GLU与3.60或7.20 μg/mL ALX联合诱导24 h，可显著增加斑马鱼GLU值，分别增加了51.52%（p<0.05）和68.32%（p<0.05）。经200、400和800 μg/mL阿卡波糖治疗处理后斑马鱼GLU值分别降低了27.28%、33.69%和38.75%，差异均显著（p<0.05），表明以10 mg/mL GLU与3.60 μg/mL ALX联合孵育24 h可以有效建立糖尿病斑马鱼模型。另外，研究发现，400和800 μg/mL CP可以显著降低10 mg/mL GLU溶液诱导的斑马鱼GLU值，分别降低了37.59%（p<0.05）和38.57%（p<0.05）；200、400和800 μg/mL CP可以显著降低糖尿病斑马鱼GLU值，分别降低了27.43%、32.75%和29.20%，差异均显著（p<0.05）。结果表明，基于正常生理和病理生理的斑马鱼生物模型评价，CP具有明显的降糖效应，其在辅助降糖功能饮品开发上具有广阔的应用前景。

In this study, 5 dpf zebrafish was used to investigate systematically zebrafish’s tolerance to glucose (GLU) and alloxan (ALX). Then, the feasibility and effectiveness of using the combination of GLU and ALX to establish a biological model of diabetic zebrafish were evaluated. On this basis, the tolerance of zebrafish to Cyclocarya paliurus (CP) was examined, and the evaluation of the hypoglycemic effect of CP was conducted. The results showed that the induction with the combination of 10 mg/mL GLU and 3.60 or 7.20 μg/mL ALX for 24 h could significantly increase the GLU levels by 51.52% (p<0.05) and 68.32% (p<0.05), respectively. The treatments with 200, 400 and 800 μg/mL acarbose decreased the GLU levels of zebrafish by 27.28% (p<0.05), 33.69% (p<0.05) and 38.75% (p<0.05), respectively, suggesting that the 24 h treatment with the combination of 10 mg/mL GLU and 3.60 μg/mL ALX could effectively establish diabetic zebrafish model. In addition, the research revealed that 400 and 800 μg/mL CP treatments could significantly decrease the GLU levels of 10 mg/mL GLU solution-treated zebrafish by 37.59% (p<0.05) and 38.57% (p<0.05), respectively. The treatments with 200, 400 and 800 μg/mL CP could significantly decrease the GLU levels by 27.43%, 32.75% (p<0.05) and 29.20% (p<0.05), respectively, in diabetic zebrafish. The results showed CP had obvious hypoglycemic effect, based on the evaluation of zebrafish with normal physiology and pathogenetic zebrafish. CP has a broad application prospect in the development of auxiliary hypoglycemic functional drinks.

浙江省公益基础研究农业项目（LGN18C200010）