为了明确1-MCP处理对软枣猕猴桃冰温（-0.5±0.3 ℃）贮藏0~60 d期间活性氧（ROS）代谢的影响，以“龙成二号”为研究试材，采用1.0 μL/L 1-甲基环丙烯（1-MCP）进行熏蒸处理，探究其对果实生理、活性氧代谢、抗坏血酸-谷胱甘肽循环的影响。结果表明：在贮藏60 d时1-MCP处理组果实呼吸强度为44.11 mg/(kg•h)，乙烯生成速率为2.35 μL/(kg•h)，分别比CK降低17.20%和23.70%，同时抑制超氧阴离子（O2-•）、过氧化氢（H2O2）、丙二醛（MDA）含量和相对电导率的升高，维持超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）活性，保持较高的还原型抗坏血酸（AsA）、氧化型谷胱甘肽（GSSG）和还原型谷胱甘肽（GSH）含量。1-MCP处理可以降低软枣猕猴桃的呼吸及乙烯生成速率，减少自由基生成，提高贮藏期的抗氧化能力，延缓果实衰老进程，保持良好的商品性，因此，该研究可以为软枣猕猴桃采后贮藏保鲜技术的完善提供理论基础和技术依据。

To analyze the effects of 1-methylcyclopropene (1-MCP) treatments on the reactive oxygen species (ROS) metabolism in Actinidia arguta stored at -0.5±0.3 ℃ for 0~60 d, the changes in the fruit physiology, ROS metabolism, and ascorbate-glutathione cycle of Actinidia arguta “Longcheng NO.2” treated with 1-MCP at 1.0 μL/L were examined. The results suggest that, at 60 d, the respiration intensity and ethylene production rate of the 1-MCP-treated fruits are 44.11 mg/(kg•h) and 2.35 μL/(kg•h), respectively. These values are 17.20% and 23.70% lower than those of the control group (CK), correspondingly. In addition, owing to the 1-MCP treatment, the increases in the levels of superoxide anion, hydrogen peroxide, and malondialdehyde and the relative conductivity were alleviated, whereas the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione reductase were well maintained. Simultaneously, the reduced ascorbic acid, oxidized glutathione, and reduced glutathione levels were maintained at relatively high levels. In summary, 1-MCP treatments can effectively reduce the respiration intensity and ethylene production rate of Actinidia arguta as well as the production of free radicals. In this manner, the antioxidant capacity during storage is enhanced and the aging of the fruits is slowed down, maintaining the commercial value of the fruits. Hence, the findings of this study can help theoretically and technically to improve the postharvest storage and preservation technology of Actinidia arguta.

国家重点研发计划项目（2018YFD0401303）