应用超高效液相色谱-离子阱-超高分辨率质谱技术对苎麻籽乙醇提取物主要化学成分进行了分析与鉴定。高效液相色谱条件为Agilent 5TC-C18色谱柱（4.6 mm×250 mm，5 μm）、0.2%甲酸水溶液-乙腈作为流动相梯度洗脱。质谱条件为电喷雾离子源（ESI），离子阱质量分析器（orbitrap），喷雾电压3500 V（正离子模式）。通过1,1-二苯基-2-吡啶并肼基-高效液相色谱（1,1-diphenyl-2-picrylhydrazyl-high performance liquid chromatography，DPPH-HPLC）方法，发现苎麻籽乙醇提取物中，存在多种抗氧化活性成分。进一步扫描相应色谱峰的紫外吸收光谱，结合标准品质谱数据、MsBank、mzCloud质谱数据库和相关文献等多种方式，解析其质谱信息，从苎麻籽乙醇提取物中共鉴别出20种化合物，其中黄酮类化合物10种，分别为原花青素二聚体、芦丁、异槲皮苷/金丝桃苷、山萘酚-3-O-β-D-芸香糖苷、槲皮素-3-O-β-D-木糖苷、山萘酚-3-O-β-D-葡萄糖苷/山萘酚-3-O-β-D-半乳糖苷、槲皮苷、槲皮素、山萘酚、阿福豆苷。其他化合物有糖类物质1种，核苷类2种，氨基酸类1种，B族维生素3种。苎麻籽乙醇提取物中存在的黄酮类化合物主要为具有抗氧化活性的黄酮苷元，表明苎麻籽有潜在的抗氧化活性。该研究为苎麻籽的进一步开发和利用提供科学依据。

The main chemical composition of the ethanol extract from ramie seeds were analyzed and identified using ultra performance liquid chcromatography-orbitrap-high resolution mass spectrometer (UPLC-Orbitrap-HRMS). UPLC conditions: Agilent 5TC-C18 chromatographic column (4.6 mm×250 mm, 5 μm), the mobile phase gradient elution with 0.2% formic acid water solution and acetonitrile. HRMS conditions: electrospray ion source (ESI), ion trap mass analyzer (orbitrap), spray voltage 3500 V (positive ion mode). The mass spectrum information was analyzed by mass spectrometry data of standards, MsBank mzCloud mass spectrometry databases and relevant literature. Twenty compounds were identified from the ethanol extract of ramie seeds. In which, there are ten flavonoids, including proanthocyanidin dimer, rutin, isoquercitrin/hyperioside, kaempferol-3-O-β-D-rutin, quercetin-3-O-β-D-xyloside, kaempferol-3-O-β-D- glucoside/kaempferol-3-O-β-D-galactoside, querceitrin, quercetin, kaempferol and afzelin. The flavonoids in the ethanol extract of ramie seeds are mainly flavonoid aglycones with antioxidant activity, indicating that ramie seeds have potential antioxidant activity. This study provides a scientific basis for the development and utilization of ramie seed.

湖南省自然科学基金面上项目（2020JJ4354）