该研究以桄榔淀粉为原料制备桄榔抗性淀粉（APRS），采用体外发酵考察不同浓度（0.1%、0.2%、0.5%、1%、1.5%）APRS对保加利亚乳杆菌（Lactobacillus bulgaricus，LB）和植物乳杆菌（Lactobacillus plantarum，LP）增殖作用及在模拟人体胃肠道逆环境中的耐受能力影响。结果表明：当APRS浓度逐渐增加到0.2%时，LB的OD达到最大值为0.91，当APRS浓度逐渐增加为0.5%时，LP的OD达到最大值0.94，但随着APRS含量的继续升高，益生菌液浓度显著降低；故低浓度下（0.1%~0.5%）APRS可促进LB和LP的生长，但高浓度（1%~1.5%）不利于LB和LP的生长；与葡萄糖（GLU）相比，APRS可显著增强LB和LP耐酸性环境、高浓度胆汁酸盐及胃液逆环境的能力（p<0.05）。APRS对益生菌的增殖作用展现出低浓度促进高浓度抑制现象，使其在胃肠道环境中耐受性增强，APRS潜在的益生作用可为其进一步开发提供理论参考。

In this study, the starch from Arenga pinnata was used as the raw material to prepare Arenga pinnata resistant starch (APRS). In vitro fermentation was performed to investigate the effects of different concentrations of APRS (0.1%, 0.2%, 0.5%, 1%, 1.5%) on the proliferation and tolerance capability in the adverse gastrointestinal environment of Lactobacillus bulgaricus (LB) and Lactobacillus plantarum (LP) were studied. The results showed that when the APRS concentration gradually increased to 0.2%, the OD600 nm of LB reached the maximum (0.91); when the APRS concentration gradually increased to 0.5%, the OD600 nm of LP reached the maximum (0.94). However, with the continuous increase of APRS content, the concentration of probiotics solution decreased significantly. Therefore, APRS at a low concentration (0.1%~0.5%) could promote on the growth of LB and LP; but APRS at a high concentration (1%~1.5%) was not conducive to on the growth of LB and LP. Compared with glucose (GLU), APRS could enhance the tolerance capability of LB and LP to an acidic environment, high concentration of bile acid salts and gastric fluid environment (p<0.05). APRS promoted and inhibited the proliferation of probiotics at a low and high concentration, respectively, thereby enhancing their tolerance in the gastrointestinal environment. The potential prebiotic effect of APRS can provide a theoretical reference for further development of APRS.

广西自然科学基金面上项目（2019GXNSFAA185014）