为解析超声处理对花生发芽过程中基因及代谢通路的变化差异，本研究以前期筛选出适宜富集白藜芦醇的花生品种为试验原料，以经超声诱导后的发芽花生（CS）为研究对象，并以未经诱导处理的发芽花生（KB）作为对照组开展试验。结果表明：共获得1104个差异表达基因（DEG），其中521个上调表达基因和583个下调基因。在差异表达基因的GO富集分析中，对富集程度明显的前20个类别做出功能分析。共得到21个不同的COG功能注释，主要参与碳水化合物的运输和代谢、翻译后修饰和蛋白质转换。有21个DEGs富集到苯丙烷类的生物合成途径，是KEGG富集分析最显著的通路。发芽花生转录本与KEGG数据库中的苯丙烷类生物合成途径比对结果表明共3个基因arahy.Tifrunner.gnm1.ann1.DXZI51、arahy.Tifrunner.gnm1.ann1.VGN2GE和arahy.Tifrunner.gnm1.ann1.Y23DM6参与发芽花生苯丙烷类物质的合成。上述结果表明超声诱导能够调控发芽花生苯丙烷类生物合成代谢途径的基因表达。

To analyze the differences in genetic and metabolic pathways during germination in peanuts, peanut varieties were pre-screened to identify those with resveratrol enrichment for further evaluation. Germinated peanuts subjected to induction with ultrasound (CS) were used as the experimental group, and uninduced germinated peanuts (KB) were used as the control group. A total of 1104 differentially expressed genes (DEGs) was obtained, including 521 upregulated genes and 583 downregulated genes. In the Gene Ontology enrichment analysis of the DEGs, functional analysis of the top 20 categories with obvious enrichment levels was performed. Twenty-one different cluster of orthologous group functional annotations were obtained, which primarily involved carbohydrate transportation and metabolism, post-translational modification, and protein conversion. There were 21 biosynthetic pathways in which DEGs were enriched in phenylpropanoids, which are the most significant pathways for Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The KEGG database showed that the genes arahy.Tifrunner.gnm1.ann1.DXZI51, arahy.Tifrunner.gnm1.ann1.VGN2GE, and arahy.Tifrunner.gnm1.ann1.Y23DM6 are involved in phenylpropanoid biosynthesis. These results indicate that ultrasound induction can regulate the expression of genes related to phenylpropanoid biosynthetic pathways in germinating peanuts, and transcriptomic changes can be determined at the molecular level in peanut buds induced by ultrasound.

辽宁省博士科研启动基金计划项目（2020-BS-038）；辽宁省“揭榜挂帅”科技攻关项目：花生全产业链提质增效关键技术（2021JH1/10400034）