从中高温大曲中筛选产糖化酶菌株，并制作霉菌麸曲，对麸曲的制作条件和酿酒性能进行测定。分离得到两株高产糖化酶菌株JQ-1、JQ-2，其鉴定结果分别为米根霉菌（Rhizopusoryzae）和卷枝毛霉菌（Mucorcircinelloides）。为了使菌株能更好的应用生产，以JQ-1、JQ-2菌株制备霉菌麸曲，制曲性能测试结果表明，以全麸皮为制曲原料，控制加水量为55%，JQ-1麸曲培养96 h，JQ-2麸曲培养72 h，干燥温度为40 ℃，该条件利于麸曲糖化酶活力的保持，所得JQ-1麸曲糖化酶活力达1510.33 U/g，JQ-2麸曲达1388.26 U/g；产酒性能测试结果表明，麸曲JQ-1所酿基酒的出酒率达44.42%，酒精度为41.22% vol，麸曲JQ-2所酿基酒的出酒率达37.32%，酒精度为35.84% vol，麸曲JQ-1的酿造性能更为突出。综合制曲和生产性能，发现菌株JQ-1和JQ-2能在酿造生产中较好的发挥其糖化作用，且酿造性能麸曲JQ-1>麸曲JQ-2。该研究以期为大曲功能微生物的开发与生产应用提供一定的指导。

Strains producing glucoamylase were screened from the high temperature Daqu, used to make mould Fuqu, and its production conditions and brewing performance were determined. Two high glucoamylase producing strains JQ-1 and JQ-2 were isolated and identified as Rhizopus oryzae and Mucor circinelloides, respectively. To make the strains more suitable for production, JQ-1 and JQ-2 were used to making mould Fuqu, The test results of Fuqu making performance showed that using whole bran as Fuqu making raw material, controlling the amount (55%) of water (JQ-1 for 96 h and JQ-2 for 72 h) and drying temperature of 40 ℃, these conditions were conducive to the maintenance of glucoamylase activity of Fuqu. The glucoamylase activity of JQ-1 Fuqu and JQ-2 Fuqu reached 1510.33 U/g and 1388.26 U/g, respectively. The results of liquor production performance test showed that the liquor yield of base liquor made by bran Fuqu JQ-1 was 44.42%, and the alcohol degree was 41.22% vol; the liquor yield of base liquor made by bran Fuqu JQ-2 was 37.32%, and the alcohol degree was 35.84% vol. The brewing performance of bran Fuqu JQ-1 was more outstanding. Based on the comprehensive Fuqu preparation and production performance results, strains JQ-1 and JQ-2 were found to play a good role in saccharification in brewing production. And in terms of brewing performance, Fuqu JQ-1>Fuqu JQ-2. This study aims to provide some guidance for the development and production applications of functional microorganisms in Daqu.

四川省科技厅应用基础研究项目（2019YJ0474）；中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2018JJ014)；酿酒生物技术及应用四川省重点实验室开放基金项目（NJ2018-02）