该研究建立了慢性有机磷中毒小鼠模型，灌胃植物乳杆菌30 d后，测定小鼠脑和血胆碱酯酶（ChE）活性、脑组织活性氧（ROS）含量、肝脏和血清总超氧化物歧化酶（T-SOD）活性、丙二醛（MDA）含量，并进行肝、脑组织病理学检查，评估植物乳杆菌对慢性有机磷中毒小鼠的解毒效果。结果表明，与模型对照组相比，灌胃植物乳杆菌使慢性有机磷中毒小鼠的ChE、T-SOD活性升高，在血的ChE，高剂量组ChE活性较模型组提高了36.13%，脑组织高剂量组ChE较模型组提高了81.13%，血清T-SOD高剂量组较模型组提高了56.93%，脑组织的T-SOD高剂量组较模型组提高了65.14%；MDA和ROS的含量下降，血清的MDA高剂量组比模型组低28.91%，肝组织的T-SOD高剂量组比模型组低16.46%，脑组织的ROS模型组ROS比高剂量组高15.92%。在灌胃后，肝细胞损伤程度明显减轻；脑神经细胞、神经元和胶质细胞形态恢复正常，细胞排列较整齐。由此得出，灌胃植物乳杆菌可部分修复慢性有机磷农药中毒引起的机体损伤，具有一定的解毒作用。

A mouse model of chronic organophosphorus poisoning was first established. Subsequently, after 30 days of oral administration of Lactobacillus plantarum fermentation broth, the cholinesterase (ChE) activity in the brain and blood, the reactive oxygen species (ROS) content in brain tissue, the total superoxide dismutase (T-SOD) activity in liver and serum, and the malondialdehyde (MDA) content in liver and brain were determined. Histopathological examination of the liver and brain tissue was carried out to evaluate the detoxification and protective effects of Lactobacillus plantarum against chronic organophosphorus poisoning in mice. Results showed that in L. plantarum Z3-treated mice, the ChE activity in the mouse blood and brain increased by 36.13% and 81.13%, respectively, and the T-SOD activity in the serum and liver increased by 56.93% and 65.14%, respectively, when compared with the model control group. On the contrary, lower MDA and ROS contents were observed in the treatment groups. The contents of MDA and ROS in the serum and the contents of T-SOD in the liver and brain were respectively decreased by 28.91%, 16.46%, and 15.92% compared to those observed in the model group. After the treatment, the damage to liver cells was significantly alleviated, the morphology of brain nerve cells, neurons, and glial cells returned to normal, and the cells attained an orderly arrangement again. It is concluded that Lactobacillus plantarum can partially repair the body damage caused by chronic organophosphorus poisoning from pesticides, and a certain degree of detoxification is confirmed.

吉林农业大学2018年省级大学生创新创业训练计划项目（2018078）