[关键词]
[摘要]
为探究云南三种特色蜂蜜挥发性物质组成和差异,采用顶空固相微萃取-气相色谱-质谱法对云南三种特色蜂蜜(苕子蜜、澳洲坚果蜜、橡胶蜜)挥发性成分进行测定,结合主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)模型进行分析。结果表明:三种蜂蜜共检测出105种挥发性物质,苕子蜜检出61种,澳洲坚果蜜检出55种,橡胶蜜检出62种。苕子蜜主要挥发性成分为醛类、酸类和醇类,含量较高的物质为正壬醛和乙酸。澳洲坚果蜜主要挥发性成分为醇类、萜烯类和醛类,含量较高的物质为顺式-氧化芳樟醇和呋喃甲酸芳樟醇氧化物,特征挥发性物质为反式芳樟醇3,7-氧化物。橡胶蜜主要挥发性成分为醛类、醇类和酸类,含量较高的物质为壬醛和反式呋喃芳樟醇氧化物。PCA和PLS-DA分析结果表明,澳洲坚果蜜、苕子蜜和橡胶蜜挥发性成分之间存在显著差异,三种蜂蜜被100%准确分类。本研究表明,苕子蜜、橡胶蜜和澳洲坚果蜜有各自独特的挥发性成分,据此构建的PCA和PLS-DA模型可以实现云南这三种特色蜂蜜的有效分类鉴别。
[Key word]
[Abstract]
In order to explore the composition and difference of the volatile components in three characteristic honeys from Yunnan province, headspace solid phase microextraction coupled to gas chromatography-mass spectrometry was used to determine the volatile components of the three honeys (Vicia villosa Roth honey, Macadamia integrifolia honey, and Hevea brasiliensis honey), combined with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The results showed that a total of one hundred and five volatile compounds were detected in the three honeys, with 61 in Vicia villosa Roth honey, 55 in Macadamia integrifolia honey, and 62 in Hevea brasiliensis honey. Aldehydes, acids and alcohols were the main volatile compounds in Vicia villosa Roth honey (n-nonanal and acetic acid had higher contents). Alcohols terpenes and aldehydes were the main volatile compounds in Macadamia integrifolia honey (cis-linalool oxide and furanoid linalool oxide had higher contents, with trans- linalool 3,7-oxide as the characteristic volatile component). Aldehydes, alcohols and acids were the main volatile compounds in Hevea brasiliensis honey (nonaldehyde and trans-linalool oxide were of higher contents). The results of PCA and PLS-DA analysis showed that there were significant differences in volatile components among Vicia villosa Roth honey, Macadamia integrifolia honey, and Hevea brasiliensis honey, and the three kinds of honey were well classified with a 100% accuracy. The research showed that Vicia villosa Roth honey, Macadamia integrifolia honey and Hevea brasiliensis honey have their own unique volatile components, and the PCA and PLS-DA models established based on these volatile compounds can realize the effective classification and identification of the three honeys in Yunan.
[中图分类号]
[基金项目]
海关总署科研项目(2019HK090)