该研究利用传统生物发酵技术生产苯乳酸，并对高产苯乳酸菌株种属、安全性以及发酵工艺进行研究。通过反相高效液相色谱法筛选得到1株高产苯乳酸菌株BLCC2-0069，该菌株发酵24 h后发酵液中苯乳酸的含量为1.26 g/L；借助菌株形态观察和16S rDNA序列鉴定，初步确定该菌株为植物乳杆菌（Lactobacillu plantarum）；该菌株培养4 h进入对数生长期，10 h进入稳定期，活菌数可达到1.78×109 cfu/mL。通过对该菌株的小鼠体内安全评价，初步确定该菌株的体内安全性。同时还对植物乳杆菌（Lactobacillus plantarum）BLCC2-0069的发酵工艺进行研究，发现在培养基中添加3.00 g/L苯丙酮酸为底物时发酵液中苯乳酸产量最高可达3.96 g/L。综上，植物乳杆菌（Lactobacillus plantarum）BLCC2-0069对小鼠无毒，具有较高生物安全性，直接发酵苯乳酸的产量可达到1.26 g/L，添加底物发酵苯乳酸产量可达到3.96 g/L。研究结果可为菌株的安全应用提供理论基础。

In this study, traditional bio-fermentation technology was used to produce phenyllactic acid, and the species, safety and fermentation process of high-yielding phenyllactic acid strains were studied. A high-yielding phenyllactic acid strain BLCC2-0069 was obtained through screening by reversed-phase high performance liquid chromatography. The content of phenyllactic acid in the fermentation broth of this strain was 1.26 g/L after 24 h of fermentation; By observations on the morphology of the strain and identification of 16S rDNA sequence of the strain, the strain was preliminarily determined as Lactobacillus plantarum; the strain entered the logarithmic growth phase after 4 hours of culture, and entered the stable phase after 10 hours, with the number of viable bacteria reaching 1.78×109 cfu/mL. Through an in vivo safety evaluation in mice, this strain was preliminarily determined safe in vivo. In the meantime, the fermentation process using Lactobacillus plantarum BLCC2-0069 was also studied, and that the yield of phenyllactic acid in the fermentation broth reached the highest (3.96 g/L) when 3.00 g/L of phenylpyruvate was added to the medium as a substrate. In summary, Lactobacillus plantarum BLCC2-0069 is non-toxic to mice and has a high level of biological safety. The yield of phenyllactic acid reached 1.26 g/L through direct fermentation, and 3.96 g/L through fermentation with a substrate addition. The results can provide a theoretical basis for the safe application of the strain.

国家重点研发计划项目（2017YFD0501000）