基于代谢组学方法研究低能离子束诱变重组菌株N6076与原始菌株Kh08在不同发酵时期的差异性胞外代谢产物，并通过网络药理学探讨了相关代谢通路。结果表明，不同时期两种菌株的胞外代谢产物存在明显差异，鉴定出VIP>1的15种代谢差异物，其中吲哚3-丙酸不仅作为共同的代谢产物，而且其差异性也最为显著。基于网络药理学的差异代谢物的通路分析，结果表明该重组菌株中有四条代谢通路存在显著性差异，分别是：醚脂代谢、糖基磷脂酰肌醇（GPI）-锚定生物合成、泛酸和CoA的生物合成、甘油磷脂。论文研究为进一步认识重组酵母菌的代谢差异提供了科学依据，为下一步的菌种改良提供可能的驯化方向。

Differences between extracellular metabolites of recombinant strain N6076 mutagenized by low-energy ion beams and original strain Kh08 at different fermentation stages were studied by metabolomic methods. The related metabolic pathways were discussed based on network pharmacology. The results show significant differences between the extracellular metabolites of the two strains at different stages. Fifteen metabolites with VIP>1 were identified including indole-3-propionate (IPA), a common metabolite showing the most significant difference. The pathways of metabolites showing differences were examined based on network pharmacology. It has been found that four metabolic pathways of the recombinant strain show significant differences, namely lipid metabolism, phosphatidylinositol (GPI)-anchoring, pantothenic acid and CoA biosynthesis, and the glycerophospholipid metabolism. This study provides scientific evidence and understanding of the metabolic differences of recombinant yeast and a possible direction for future strain improvement.

国家自然科学基金地区基金项目（31760016）；陕西省重点研发计划项目（2019GY-138；2020GY-236）；陕西省中医药管理局科研项目（2019-ZZ-ZY005）