本研究通过小鼠实验和菌群体外增殖实验探究复合代糖的通便功效及其对肠道菌群的调节作用。将Balb/c小鼠分为空白对照组、模型组、阳性对照组和复合代糖干预G1组（0.75 g/kg·BW）、G2组（1.5 g/kg·BW）、G3组（2.25 g/kg·BW）、G4组（3 g/kg·BW）、G5组（3.75 g/kg·BW），用洛哌丁胺建立便秘模型，酚酞和复合代糖进行干预，14 d后观察墨汁推进率、首粒黑便时间、6 h的排便粒数和6 h粪便重量等指标，评价复合代糖的通便效果。并通过体外培养实验探究复合代糖对小鼠肠道菌群的增殖影响。结果表明，五种剂量的复合代糖干预组均可促进小鼠排便，其中3.75 g/kg·BW剂量的通便功效最为显著，相对于模型组，墨汁推进率增加了48.47%、首粒黑便时间减少了67.25 min、6 h排便粒数增加了8.75 n、6 h粪便重量增加了0.20 g。体外培养实验发现0.075 g/mL、0.15 g/mL、0.225 g/mL、0.3 g/mL、0.375 g/mL浓度的复合代糖可促进双歧杆菌、乳酸杆菌的增殖和抑制肠杆菌、肠球菌的增殖，并在0.075 g/mL至0.375 g/mL浓度内，浓度越大，促进双歧杆菌、乳酸杆菌增殖的趋势越明显。本研究的复合代糖具有通便效果，对肠道菌群有调节作用。

The laxative effect and the regulation of intestinal flora of complex sugar substitutes were investigated by mice experiment and in vitro bacterial proliferation experiment in this study. To evaluate the laxative effect of compound substitute sugar, Balb/C mice were divided into blank control group, model group, positive control group, compound glucose-substituted G1 group (0.75 g/kg·BW), G2 group (1.5 g/kg·BW), G3 group (2.25 g/kg·BW), G4 group (3 g/kg·BW) and G5 group (3.75 g/kg·BW) to establish constipation model with loperamide, and phenolphthalein and five different concentrations of complex substitute sugars were used for intervention. The weight of mice before and after the experiment, the advance rate of ink, the time of the first black defecation, the number of defecation grains within 6 h and the weight of defecation were observed. The effect of compound sugars on the proliferation of intestinal flora in mice was explored through in vitro culture experiments. The results showed that five different doses of compound sugar substitute intervention group could promote the defecation of mice, among which the defecation effect of 3.75 g/kg·BW group was the most significant. Compared with the model group, the ink propulsion rate increased by 48.47%, the first black stool time decreased by 67.25 min, the number of defecation particles increased by 8.75 n in 6 h, and the fecal weight increased by 0.20 g in 6 h. The in vitro culture experiment showed that compound sugar at the concentration of 0.75 g/mL, 0.15 g/mL, 0.225 g/mL, 0.3 g/mL and 0.375 g/mL could promote the proliferation of Bifidobacterium and Lactobacillus and inhibit the proliferation of Enterobacter and Enterococcus. In the concentration from 0.075 g/mL to 0.375 g/mL, the higher the concentration of compound sugar, the more obvious the trend of promoting the proliferation of Bifidobacterium and Lactobacillus. Therefore, the compound sugar substitute in this study has a laxative effect and can regulate the intestinal flora.

国家自然科学基金项目（81773428；81703219）