[关键词]
[摘要]
本研究运用气相色谱-质谱联用(GC-MS)和液相色谱-串联质谱(LC-MS/MS)广泛代谢组手段全面分析慈竹笋的营养成分组成,并从清除自由基、相对还原力、总抗氧化能力、羟自由基清除能力和超氧自由基清除能力方面系统研究其抗氧化活性。结果表明,GC-MS代谢组共鉴定到75种代谢物,其中含量超过10%的主要为糖类物质,如葡萄糖(34.98%)、果糖(19.18%)和蔗糖(14.99%),且另有9种代谢物的相对含量大于1%。LC-MS/MS代谢组共检测出226种代谢物,相对含量最高的是有机酸,16种代谢物相对含量大于0.5%,其中相对含量大于1%的代谢物共有9种。抗氧化活性结果表明,慈竹笋提物含量与自由基清除能力、相对还原能力、总抗氧化能力成正相关,其中1 mg/mL提取物DPPH自由基清除率为82.70%,羟自由基清除率为89.70%,超氧自由基清除率为43.80%,相对还原力为27.90%,FRAP值为100.10 μmol/L。基于慈竹笋富含糖类、有机酸、氨基酸等成分并表现出较高的抗氧化活性,因而在加工成功能性食品上具有很大的前景。
[Key word]
[Abstract]
In the present study, the nutritional components of Bambusa emeiensisi shoots were analyzed by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS), and the antioxidant activity was systematically investigated by elimination of DPPH?, O2-?and OH?, reducing power and total oxidation resistance. The results showed that a total of 75 metabolites were identified in GC-MS metabolome, among which the main metabolites with more than 10% content were carbohydrate, such as glucose (34.98%), fructose (19.18%) and sucrose (14.99%), and the relative contents of another 9 metabolites were more than 1%. A total of 226 metabolites were detected in LC-MS/MS metabolome, the highest relative content of which was organic acids. The relative content of 16 metabolites was greater than 0.5%, and there were 9 metabolites whose relative content was greater than 1%. The antioxidant activity results showed that the content of the extracts was positively correlated with the free radical scavenging capacity, relative reducing capacity and total antioxidant capacity, among which the DPPH scavenging rate of the 1 mg/mL extract was 82.70%, the hydroxyl radical scavenging rate was 89.70%, the superoxide radical scavenging rate was 43.80%, the relative reducing power was 27.90%, and the FRAP value was 100.10 mol/L. B. emeiensisi shoots are rich in sugars, organic acids, amino acids and other components and exhibit high antioxidant activity, so they have a great prospect in the processing of functional food.
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[基金项目]
四川省科技计划项目(2019YGF0239);竹类病虫防控与资源开发四川省重点实验室开放项目(ZLKF1902);乐山师范学院科技资助项目(TDZZ202003)